Szczegóły obiektu: The Ability of Lytic Staphylococcal Podovirus vB_SauP_phiAGO1.3 to Coexist in Equilibrium With Its Host Facilitates the Selection of Host Mutants of Attenuated Virulence but Does Not Preclude the Phage Antistaphylococcal Activity in a Nematode Infection Model

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Tytuł:
The Ability of Lytic Staphylococcal Podovirus vB_SauP_phiAGO1.3 to Coexist in Equilibrium With Its Host Facilitates the Selection of Host Mutants of Attenuated Virulence but Does Not Preclude the Phage Antistaphylococcal Activity in a Nematode Infection Model
Twórca:
Głowacka-Rutkowska, Aleksandra;
Gozdek, Agnieszka;
Empel, Joanna;
Gawor, Jan;
Żuchniewicz, Karolina;
Kozińska, Aleksandra;
Dębski, Janusz;
Gromadka, Robert;
Łobocka, Małgorzata
Opis:
Phage vB_SauP_phiAGO1.3 (phiAGO1.3) is a polyvalent Staphylococcus lytic podovirus with a 17.6-kb genome (Gozdek et al., 2018). It can infect most of the Staphylococcus aureus human isolates of dominant clonal complexes. We show that a major factor contributing to the wide host range of phiAGO1.3 is a lack or sparcity of target sites for certain restriction-modification systems of types I and II in its genome. Phage phiAGO1.3 requires for adsorption β-O-GlcNAcylated cell wall teichoic acid, which is also essential for the expression of methicillin resistance. Under certain conditions an exposure of S. aureus to phiAGO1.3 can lead to the establishment of a mixed population in which the bacteria and phages remain in equilibrium over multiple generations. This is reminiscent of the so called phage carrier state enabling the co-existence of phage-resistant and phage-sensitive cells supporting a continuous growth of the bacterial and phage populations. The stable co-existence of bacteria and phage favors the emergence of phage-resistant variants of the bacterium. All phiAGO1.3-resistant cells isolated from the phage-carrier-state cultures contained a mutation inactivating the two-component regulatory system ArlRS, essential for efficient expression of numerous S. aureus virulence-associated traits. Moreover, the mutants were unaffected in their susceptibility to infection with an unrelated, polyvalent S. aureus phage of the genus Kayvirus. The ability of phiAGO1.3 to establish phage-carrier-state cultures did not preclude its antistaphylococcal activity in vivo in an S. aureus nematode infection model. Taken together our results suggest that phiAGO1.3 could be suitable for the therapeutic application in humans and animals, alone or in cocktails with Kayvirus phages. It might be especially useful in the treatment of infections with the majority of methicillin-resistant S. aureus strains.
Dostępność obiektu:
Dostęp otwarty
Data:
2019.01.18
Typ:
Article;
PeerReviewed
Język:
angielski
Format:
application/pdf
Relacja: http://eprints.ibb.waw.pl/1697 ;https://www.frontiersin.org/journals/microbiology# ;doi: 10.3389/fmicb.2018.03227
Temat i słowa kluczowe:
QH426 Genetics;
QR180 Immunology;
QR355 Virology
Identyfikator: http://eprints.ibb.waw.pl/1697/1/fmicb-09-03227.pdf ;
Głowacka-Rutkowska, Aleksandra and Gozdek, Agnieszka and Empel, Joanna and Gawor, Jan and Żuchniewicz, Karolina and Kozińska, Aleksandra and Dębski, Janusz and Gromadka, Robert and Łobocka, Małgorzata (2019) The Ability of Lytic Staphylococcal Podovirus vB_SauP_phiAGO1.3 to Coexist in Equilibrium With Its Host Facilitates the Selection of Host Mutants of Attenuated Virulence but Does Not Preclude the Phage Antistaphylococcal Activity in a Nematode Infection Model. Frontiers in Microbiology, 9 . p. 3227. ISSN 1664-302X
Dostawca danych:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Podobne obiekty

The Ability of Lytic Staphylococcal Podovirus vB_SauP_phiAGO1.3 to Coexist in Equilibrium With Its Host Facilitates the Selection of Host Mutants of Attenuated Virulence but Does Not Preclude the Phage Antistaphylococcal Activity in a Nematode Infection Model

Data:2019.01.18

Rodzaj zawartości:pozostałe

A prime/boost vaccination with HA DNA and Pichia -produced HA protein elicits a strong humoral response in chickens against H5N1

Data:2017.01.31

Rodzaj zawartości:pozostałe

Immunogenicity of DNA Vaccine against H5N1 Containing Extended Kappa B Site: In Vivo Study in Mice and Chickens

Data:2017.08.24

Rodzaj zawartości:pozostałe

Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris.

Data:2014

Rodzaj zawartości:pozostałe

Virus-like particles as vaccine.

Twórca:Chroboczek, Jadwiga | Szurgot, Inga | Szolajska, Ewa

Data:2014

Rodzaj zawartości:pozostałe

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Data:2021.08.19

Rodzaj zawartości:pozostałe

Phage Therapy vs. the Use of Antibiotics in the Treatment of Salmonella-Infected Chickens: Comparison of Effects on Hematological Parameters and Selected Biochemical Markers

Twórca:Grabowski, Łukasz | Węgrzyn, Grzegorz | Węgrzyn, Alicja | Podlacha, Magdalena

Data:2022.12.09

Rodzaj zawartości:pozostałe

Highly different effects of phage therapy and antibiotic therapy on immunological responses of chickens infected with Salmonella enterica serovar Typhimurium

Twórca:Grabowski, Łukasz | Węgrzyn, Grzegorz | Węgrzyn, Alicja | Podlacha, Magdalena

Data:2022.09.23

Rodzaj zawartości:pozostałe

Federacja Bibliotek Cyfrowych

Szczegóły obiektu: The Ability of Lytic Staphylococcal Podovirus vB_SauP_phiAGO1.3 to Coexist in Equilibrium With Its Host Facilitates the Selection of Host Mutants of Attenuated Virulence but Does Not Preclude the Phage Antistaphylococcal Activity in a Nematode Infection Model

Opis

Podobne obiekty