Opis

• Tytuł:
  The Non-Lantibiotic Bacteriocin Garvicin Q Targets Man-PTS in a Broad Spectrum of Sensitive Bacterial Genera
• Twórca:
  Tymoszewska, Aleksandra;
  Diep, Dzung B.;
  Wirtek, Paulina;
  Aleksandrzak-Piekarczyk, Tamara
• Opis:
  Mannose phosphotransferase system (Man-PTS) is the main mannose permease in bacteria but it is also a known receptor for subclass IIa bacteriocins (pediocin-like group) as well as subclass IId lactococcin A (LcnA) and lactococcin B (LcnB) (LcnA-like group). Subclass IIa bacteriocins exhibit a strong activity against Listeria spp. but they are not against Lactococcus spp. In contrast, the LcnA-like bacteriocins act only against Lactococcus lactis strains. Garvicin Q (GarQ) is a subclass IId bacteriocin with minor similarity to LcnA-like bacteriocins and a relatively broad antimicrobial spectrum including, among others, Listeria and Lactococcus spp. To identify the GarQ receptor, we obtained GarQ-resistant mutants of Lactococcus garvieae IBB3403 and L. lactis IL1403 and sequenced their genomes that revealed mutations in genes encoding the membrane-bound Man-PTS IIC or IID subunits encoded by ptnCD in L. lactis and manCD in L. garvieae. This is the first time that a bacteriocin outside the pediocin- and LcnA-like groups is shown to target Man-PTS. The interaction between GarQ and Man-PTS may occur through a new binding pattern involving specific amino acids highly conserved among the GarQ-sensitive bacterial species located in the N-terminal part and extracellular loops of subunit IID and in transmembrane region of IIC.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2017
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski;
  eng
• Format:
  text/html
• Relacja: http://eprints.ibb.waw.pl/1386 ;http://doi.org/10.1038/s41598-017-09102-7 ;doi:10.1038/s41598-017-09102-7
• Temat i słowa kluczowe:
  QR Microbiology
• Identyfikator: http://eprints.ibb.waw.pl/1386/1/s41598-017-09102-7 ;
  Tymoszewska, Aleksandra and Diep, Dzung B. and Wirtek, Paulina and Aleksandrzak-Piekarczyk, Tamara (2017) The Non-Lantibiotic Bacteriocin Garvicin Q Targets Man-PTS in a Broad Spectrum of Sensitive Bacterial Genera. Scientific Reports, 7 (1). ISSN 2045-2322
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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