Opis

• Tytuł:
  Configuration of polyisoprenoids affects the permeability and thermotropic properties of phospholipid/polyisoprenoid model membranes.
• Twórca:
  Ciepichał-Głodowska, E.;
  Jemioła-Rzemińska, M.;
  Hertel, J.;
  Swiezewska, Ewa;
  Strzałka, K.
• Opis:
  The influence of α-cis- and α-trans-polyprenols on the structure and properties of model membranes was analyzed. The interaction of Ficaprenol-12 (α-cis-Prenol-12, α-Z-Prenol-12) and Alloprenol-12 (α-trans-Prenol-12, α-E-Prenol-12) with model membranes was compared using high performance liquid chromatography (HPLC), differential scanning calorimetry (DSC) and fluorescent methods. L-α-phosphatidylcholine from egg yolk (EYPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as the main lipid components of unilamellar (SUVs) and multilamellar (MLVs) vesicles were used. The two-step extraction procedure (n-pentane and hexane, respectively) allowed to separately analyze the fractions of polyprenol as non-incorporated (PrenolNonInc) and incorporated (PrenolInc) into liposomes. Consequently, distribution coefficients, P', describing the equilibrium of prenol content between phospholipid (EYPC) membrane and the aqueous phase gave different logP' for α-cis- and α-trans-Prenol-12, indicating that the configuration of the α-terminal residue significantly alters the hydrophobicity of the polyisoprenoid molecule and consequently the affinity of polyprenols for EYPC membrane. In fluorescence experiments α-trans-Pren-12 increased up to 1.7-fold the permeability of EYPC bilayer for glucose while the effect of α-cis-Pren-12 was almost negligible. Considerable changes of thermotropic behavior of DPPC membranes in the presence of both prenol isomers were observed. α-trans-Pren-12 completely abolished the pretransition while in the case of α-cis-Pren-12 it was noticeably reduced. Furthermore, for both prenol isomers, the temperature of the main phase transition (Tm) was shifted by about 1ºC to lower values and the height of the peak was significantly reduced. The DSC analysis profiles also showed a new peak at 38.7°C, which may suggest the concomitant presence of more that one phase within the membrane. Results of these experiments and the concomitant occurrence of alloprenols and ficaprenols in plant tissues suggest that cis/trans isomerization of the α-residue of polyisoprenoid molecule might comprise a putative mechanism responsible for modulation of the permeability of cellular membranes.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2011
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski
• Format:
  application/msword
• Wydawca:
  Elsevier
• Relacja: http://eprints.ibb.waw.pl/133
• Temat i słowa kluczowe:
  QK Botany
• Identyfikator: http://eprints.ibb.waw.pl/133/1/Chem_Phys_Lipids_manuscript_revised.doc ;
  Ciepichał-Głodowska, E. and Jemioła-Rzemińska, M. and Hertel, J. and Swiezewska, Ewa and Strzałka, K. (2011) Configuration of polyisoprenoids affects the permeability and thermotropic properties of phospholipid/polyisoprenoid model membranes. Chemistry and Physics of Lipids, 164 . p. 300. (In Press)
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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