Opis

• Tytuł:
  U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein
• Twórca:
  Skrajna, Aleksandra;
  Yang, XC;
  Bucholc, Katarzyna;
  Zhang, J;
  Hall, TMT;
  Dadlez, Michal;
  Marzluff, WF;
  Dominski, Z
• Opis:
  Cleavage of histone pre-mRNAs at the 3' end requires stem-loop binding protein (SLBP) and U7 snRNP that consists of U7 snRNA and a unique Sm ring containing two U7-specific proteins: Lsm10 and Lsm11. Lsm11 interacts with FLASH and together they bring a subset of polyadenylation factors to U7 snRNP, including the CPSF73 endonuclease that cleaves histone pre-mRNA. SLBP binds to a conserved stem-loop structure upstream of the cleavage site and acts by promoting an interaction between the U7 snRNP and a sequence element located downstream from the cleavage site. We show that both human and Drosophila SLBPs stabilize U7 snRNP on histone pre-mRNA via two regions that are not directly involved in recognizing the stem-loop structure: helix B of the RNA binding domain and the C-terminal region that follows the RNA binding domain. Stabilization of U7 snRNP binding to histone pre-mRNA by SLBP requires FLASH but not the polyadenylation factors. Thus, FLASH plays two roles in 3' end processing of histone pre-mRNAs: It interacts with Lsm11 to form a docking platform for the polyadenylation factors, and it cooperates with SLBP to recruit U7 snRNP to histone pre-mRNA.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2017.03.13
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski;
  eng
• Format:
  application/pdf
• Wydawca:
  Cold Spring Harbor Laboratory Press:
• Relacja: http://eprints.ibb.waw.pl/1692 ;https://rnajournal.cshlp.org/content/23/6/938.long ;
  10.1261/rna.060806.117
• Temat i słowa kluczowe:
  Q Science (General)
• Identyfikator: http://eprints.ibb.waw.pl/1692/1/Publikacja.pdf ;
  Skrajna, Aleksandra and Yang, XC and Bucholc, Katarzyna and Zhang, J and Hall, TMT and Dadlez, Michal and Marzluff, WF and Dominski, Z (2017) U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein. RNA, 23 (6). pp. 938-951.
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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