Opis

• Tytuł:
  Identification and functional characterization of the Arabidopsis Snf1-related protein kinase SnRK2.4 phosphatidic acid-binding domain.
• Twórca:
  Julkowska, Magdalena M;
  McLoughlin, Fionn;
  Galvan-Ampudia, Carlos S;
  Rankenberg, Johanna M;
  Kawa, Dorota;
  Klimecka, Maria;
  Haring, Michel A;
  Munnik, Teun;
  Kooijman, Edgar E;
  Testerink, Christa
• Opis:
  Phosphatidic acid (PA) is an important signalling lipid involved in various stress-induced signalling cascades. Two SnRK2 protein kinases (SnRK2.4 and SnRK2.10), previously identified as PA-binding proteins, are shown here to prefer binding to PA over other anionic phospholipids and to associate with cellular membranes in response to salt stress in Arabidopsis roots. A 42 amino acid sequence was identified as the primary PA-binding domain (PABD) of SnRK2.4. Unlike the full-length SnRK2.4, neither the PABD-YFP fusion protein nor the SnRK2.10 re-localized into punctate structures upon salt stress treatment, showing that additional domains of the SnRK2.4 protein are required for its re-localization during salt stress. Within the PABD, five basic amino acids, conserved in class 1 SnRK2s, were found to be necessary for PA binding. Remarkably, plants overexpressing the PABD, but not a non-PA-binding mutant version, showed a severe reduction in root growth. Together, this study biochemically characterizes the PA-SnRK2.4 interaction and shows that functionality of the SnRK2.4 PABD affects root development.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2015.07.30
• Typ:
  Article;
  PeerReviewed
• Relacja: http://eprints.ibb.waw.pl/852 ;
  10.1111/pce.12421
• Temat i słowa kluczowe:
  QK Botany;
  QP Physiology
• Identyfikator:
  Julkowska, Magdalena M and McLoughlin, Fionn and Galvan-Ampudia, Carlos S and Rankenberg, Johanna M and Kawa, Dorota and Klimecka, Maria and Haring, Michel A and Munnik, Teun and Kooijman, Edgar E and Testerink, Christa (2015) Identification and functional characterization of the Arabidopsis Snf1-related protein kinase SnRK2.4 phosphatidic acid-binding domain. Plant, cell & environment, 38 (3). pp. 614-624. ISSN 1365-3040
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Czy mogę z tego obiektu skorzystać?:
  poproś o pozwolenie
• Rodzaj zawartości:
  pozostałe