Opis

• Tytuł:
  Biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage
• Twórca:
  Jurczak-Kurek, Agata;
  Gąsior, Tomasz;
  Nejman-Faleńczyk, Bożena;
  Bloch, Sylwia;
  Dydecka, Aleksandra;
  Topka, Gracja;
  Necel, Agnieszka;
  Jakubowska-Deredas, Magdalena;
  Narajczyk, Magdalena;
  Richert, Malwina;
  Mieszkowska, Agata;
  Wróbel, Borys;
  Węgrzyn, Grzegorz;
  Węgrzyn, Alicja
• Opis:
  A large scale analysis presented in this article focuses on biological and physiological variety of bacteriophages. A collection of 83 bacteriophages, isolated from urban sewage and able to propagate in cells of different bacterial hosts, has been obtained (60 infecting Escherichia coli, 10 infecting Pseudomonas aeruginosa, 4 infecting Salmonella enterica, 3 infecting Staphylococcus sciuri, and 6 infecting Enterococcus faecalis). High biological diversity of the collection is indicated by its characteristics, both morphological (electron microscopic analyses) and biological (host range, plaque size and morphology, growth at various temperatures, thermal inactivation, sensitivity to low and high pH, sensitivity to osmotic stress, survivability upon treatment with organic solvents and detergents), and further supported by hierarchical cluster analysis. By the end of the research no larger collection of phages from a single environmental source investigated by these means had been found. The finding was confirmed by whole genome analysis of 7 selected bacteriophages. Moreover, particular bacteriophages revealed unusual biological features, like the ability to form plaques at low temperature (4 °C), resist high temperature (62 °C or 95 °C) or survive in the presence of an organic solvents (ethanol, acetone, DMSO, chloroform) or detergent (SDS, CTAB, sarkosyl) making them potentially interesting in the context of biotechnological applications.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2016
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski;
  eng
• Format:
  application/pdf
• Wydawca:
  Macmillan Publishers Limited
• Relacja: http://eprints.ibb.waw.pl/1308 ;http://www.nature.com/articles/srep34338
• Temat i słowa kluczowe:
  QH301 Biology;
  QH426 Genetics;
  QR Microbiology;
  QR355 Virology
• Identyfikator: http://eprints.ibb.waw.pl/1308/1/AW_3.pdf ;
  Jurczak-Kurek, Agata and Gąsior, Tomasz and Nejman-Faleńczyk, Bożena and Bloch, Sylwia and Dydecka, Aleksandra and Topka, Gracja and Necel, Agnieszka and Jakubowska-Deredas, Magdalena and Narajczyk, Magdalena and Richert, Malwina and Mieszkowska, Agata and Wróbel, Borys and Węgrzyn, Grzegorz and Węgrzyn, Alicja (2016) Biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage. Scientific Reports, 6 (34338). ISSN 2045-2322
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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