Object's details: Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis
Creator:
Lipko, Agata;
Pączkowski, Cezary;
Perez-Fons, Laura;
Fraser, Paul D.;
Kania, Magdalena;
Hoffman-Sommer, Marta;
Danikiewicz, Witold;
Rohmer, Michel;
Poznanski, Jaroslaw;
Swiezewska, Ewa
Description:
Isoprenoids, including dolichols (Dols) and polyprenols (Prens), are ubiquitous components of eukaryotic cells. In plant cells, there are two pathways that produce precursors utilized for isoprenoid biosynthesis: the mevalonate (MVA) pathway and the methylerythritol phosphate (MEP) pathway. In this work, the contribution of these two pathways to the biosynthesis of Prens and Dols was addressed using an in planta experimental model. Treatment of plants with pathway-specific inhibitors and analysis of the effects of various light conditions indicated distinct biosynthetic origin of Prens and Dols. Feeding with deuteriated, pathway-specific precursors revealed that Dols, present in leaves and roots, were derived from both MEP and MVA pathways and their relative contributions were modulated in response to precursor availability. In contrast, Prens, present in leaves, were almost exclusively synthesized via the MEP pathway. Furthermore, results obtained using a newly introduced here ‘competitive’ labeling method, designed so as to neutralize the imbalance of metabolic flow resulting from feeding with a single pathway-specific precursor, suggest that under these experimental conditions one fraction of Prens and Dols is synthesized solely from endogenous precursors (deoxyxylulose or mevalonate), while the other fraction is synthesized concomitantly from endogenous and exogenous precursors. Additionally, this report describes a novel methodology for quantitative separation of 2H and 13C distributions observed for isotopologues of metabolically labeled isoprenoids. Collectively, these in planta results show that Dol biosynthesis, which uses both pathways, is significantly modulated depending on pathway productivity, while Prens are consistently derived from the MEP pathway.
Object availability:
free access
Date:
2023
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
Portland Press
Relation: http://eprints.ibb.waw.pl/2278 ;https://portlandpress.com/biochemj/article/480/8/495/232868/Divergent-contribution-of-the-MVA-and-MEP-pathways ;doi.org/10.1042/BCJ20220578
Subject:
QH301 Biology
Identifier: http://eprints.ibb.waw.pl/2278/1/2023_BCJ_Lipko.pdf ;
Lipko, Agata and Pączkowski, Cezary and Perez-Fons, Laura and Fraser, Paul D. and Kania, Magdalena and Hoffman-Sommer, Marta and Danikiewicz, Witold and Rohmer, Michel and Poznanski, Jaroslaw and Swiezewska, Ewa (2023) Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis. Biochemical Journal, 480 (8). pp. 495-520. ISSN 0264-6021
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis

Date:2023

Type:other

LTR retrotransposons in fungi.

Creator:Muszewska, Anna | Hoffman-Sommer, Marta | Grynberg, Marcin

Date:2011

Type:other

The C-terminal domain of the Salmonella enterica WbaP (UDP-galactose:Und-P galactose-1-phosphate transferase) is sufficient for catalytic activity and specificity for undecaprenyl monophosphate

Creator:Patel, Kinnari | Ciepichal, Ewa | Swiezewska, Ewa | Valvano, Miguel

Date:2012

Type:other

SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder.

Creator:Swiezewska, Ewa

Date:2010.07.23

Type:other

Preference of bacterial rhamnosyltransferases for 6‑deoxysugars reveals a strategy to deplete O‑antigens

Date:2023

Type:other

NMR of carbohydrates, lipids and membranes

Creator:Swiezewska, Ewa | Wójcik, Jacek

Date:2011

Type:other

A cytosolic bifunctional geranyl/farnesyl diphosphate synthase provides MVA-derived GPP for geraniol biosynthesis in rose flowers

Date:2023

Type:other

The history and recent advances in research of polyprenol and its derivatives

Creator:Sagami, Hiroshi | Swiezewska, Ewa | Shidoji, Yoshihiro

Date:2018

Type:other

Federacja Bibliotek Cyfrowych

Object's details: Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis

Description

Similar objects