Federacja Bibliotek Cyfrowych

Homepage
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae.

Object's details: Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae.

tile.noImage

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae.
Creator:
Jaszczur, Małgorzata;
Flis, Krzysztof;
Rudzka, Justyna;
Kraszewska, Joanna;
Budd, Martin E;
Polaczek, Piotr;
Campbell, Judith L;
Jonczyk, Piotr;
Fijalkowska, Iwona J.
Description:
Most replicases are multi-subunit complexes. DNA polymerase epsilon from Saccharomyces cerevisiae is composed of four subunits: Pol2p, Dpb2p, Dpb3p, and Dpb4p. Pol2p and Dpb2p are essential. To investigate a possible role for the Dpb2p subunit in maintaining the fidelity of DNA replication, we isolated temperature-sensitive mutants in the DPB2 gene. Several of the newly isolated dpb2 alleles are strong mutators, exhibiting mutation rates equivalent to pol2 mutants defective in the 3' --> 5' proofreading exonuclease (pol2-4) or to mutants defective in mismatch repair (msh6). The dpb2 pol2-4 and dpb2 msh6 double mutants show a synergistic increase in mutation rate, indicating that the mutations arising in the dpb2 mutants are due to DNA replication errors normally corrected by mismatch repair. The dpb2 mutations decrease the affinity of Dpb2p for the Pol2p subunit as measured by two-hybrid analysis, providing a possible mechanistic explanation for the loss of high-fidelity synthesis. Our results show that DNA polymerase subunits other than those housing the DNA polymerase and 3' --> 5' exonuclease are essential in controlling the level of spontaneous mutagenesis and genetic stability in yeast cells.
Object availability:
free access
Date:
2008.02
Type:
Article;
PeerReviewed
Relation: http://eprints.ibb.waw.pl/394 ;http://www.genetics.org/content/178/2/633.long ;doi: 10.1534/genetics.107.082818
Subject:
Q Science (General);
QH Natural history;
QH301 Biology;
QH426 Genetics
Identifier:
Jaszczur, Małgorzata and Flis, Krzysztof and Rudzka, Justyna and Kraszewska, Joanna and Budd, Martin E and Polaczek, Piotr and Campbell, Judith L and Jonczyk, Piotr and Fijalkowska, Iwona J. (2008) Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae. Genetics, 178 (2). pp. 633-47. ISSN 0016-6731
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

tile.noImage

Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae.

Creator:Jaszczur, Małgorzata | Flis, Krzysztof | Rudzka, Justyna | Kraszewska, Joanna | Budd, Martin E | Polaczek, Piotr | Campbell, Judith L | Jonczyk, Piotr | Fijalkowska, Iwona J.

Date:2008.02

Type:other

tile.noImage

Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase epsilon, contributes to a mutator phenotype in Saccharomyces cerevisiae.

Creator:Jaszczur, Małgorzata | Rudzka, Justyna | Kraszewska, Joanna | Flis, Krzysztof | Polaczek, Piotr | Campbell, Judith L | Fijalkowska, Iwona J. | Jonczyk, Piotr

Date:2009.10.02

Type:other

tile.noImage

Effects of flavonoids on expression of genes involved in cell cycle regulation and DNA replication in human fibroblasts

Creator:Moskot, Marta | Jakóbkiewicz-Banecka, Joanna | Smolińska, Elwira | Piotrowska, Ewa | Węgrzyn, Grzegorz | Gabig-Cimińska, Magdalena

Date:2015

Type:other

tile.noImage

Activities of genes controlling sphingolipid metabolism in human fibroblasts treated with flavonoids

Creator:Moskot, Marta | Jakóbkiewicz-Banecka, Joanna | Smolińska, Elwira | Banecki, Bogdan | Węgrzyn, Grzegorz | Gabig-Cimińska, Magdalena

Date:2015

Type:other

tile.noImage

Effect of silicone on the collagen fibrillogenesis and stability

Creator:Kadziński, Leszek | Prokopowicz, Magdalena | Jakóbkiewicz-Banecka, Joanna | Gabig-Cimińska, Magdalena | Łukasiak, Jerzy | Banecki, Bogdan

Date:2015

Type:other

tile.noImage

Regulation of the switch from early to late bacteriophage lambda DNA replication

Creator:Barańska, Sylwia | Gabig, Magdalena | Węgrzyn, Grzegorz | Konopa, Grażyna | Herman-Antosiewicz, Anna | Hernandez, Pablo | Schvartzman, Jorge | Helinski, Donald

Date:2001

Type:other

tile.noImage

Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids

Creator:Moskot, Marta | Jakóbkiewicz-Banecka, Joanna | Kloska, Anna | Smolińska, Elwira | Mozolewski, Paweł | Malinowska, Marcelina | Rychłowski, Michał | Banecki, Bogdan | Węgrzyn, Grzegorz | Gabig-Cimińska, Magdalena

Date:2015

Type:other

tile.noImage

Electric chips for rapid detection and quantification of nucleic acids

Creator:Gabig-Cimińska, Magdalena | Holmgren, Anders | Andresen, Heiko | Barken, Kim Bundvig | Wumpelmann, M | Albers, Joerg | Hintsche, Rainer | Breitenstein, Antje | Neubauer, Peter | Łoś, Marcin | Czyż, Agata | Węgrzyn, Grzegorz | Silfvesparre, Gustav | Jurgen, Britta | Schweder, Thomas | Enfors, Sven-Olof

Date:2004

Type:other

FBC TeNe Project

Project co-financed by European Funds under the Operational Program Digital Poland for 2014-2020 (project no. POPC.02.04.00-00-0012 / 20-00)

More information about the project can be found at: https://tene.fbc.pionier.net.pl

European Funds. Digital Poland

Republic of Poland

Digital Libraries Federation

European Union - European Regional Development Fund

We use files, through the cookie quality improvement layer of our website.For more information, please read the document Privacy Policy