Object's details: Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.
Creator:
Bartosik, Aneta A;
Glabski, Krzysztof;
Jecz, Paulina;
Lasocki, Krzysztof;
Mikosa, Malgorzata;
Plochocka, Danuta;
Thomas, Christopher M.;
Jagura-Burdzy, Grazyna
Description:
Pseudomonas aeruginosa ParA belongs to a large subfamily of Walker-type ATPases acting as partitioning proteins in bacteria. ParA has the ability to both self-associate and interact with its partner ParB. Analysis of the deletion mutants defined the part of the protein involved in dimerization and interactions with ParB. Here, a set of ParA alanine substitution mutants in the region between E67 and L85 was created and analysed in vivo and in vitro. All mutants impaired in dimerization (substitutions at positions M74, H79, Y82 and L84) were also defective in interactions with ParB, suggesting that ParA-ParB interactions depend on the ability of ParA to dimerize. Mutants with alanine substitutions at positions E67, C68, L70, E72, F76, Q83 and L85 were not impaired in dimerization, but were defective in interactions with ParB. The dimerization interface partly overlapped the pseudo-hairpin, involved in interactions with ParB. ParA mutant derivatives tested in vitro showed no defects in ATPase activity. Two parA alleles (parA84, whose product can neither self-interact nor interact with ParB, and parA67, whose product is impaired in interactions with ParB, but not in dimerization) were introduced into the P. aeruginosa chromosome by homologous gene exchange. Both mutants showed defective separation of ParB foci, but to different extents. Only PAO1161 parA84 was visibly impaired in terms of chromosome segregation, growth rate and motility, similar to a parA-null mutant.
Object availability:
free access
Date:
2014.11
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/839 ;http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219104
Subject:
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/839/2/2406.full.pdf ;
Bartosik, Aneta A and Glabski, Krzysztof and Jecz, Paulina and Lasocki, Krzysztof and Mikosa, Malgorzata and Plochocka, Danuta and Thomas, Christopher M. and Jagura-Burdzy, Grazyna (2014) Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB. Microbiology (Reading, England), 160 (Pt 11). pp. 2406-20. ISSN 1465-2080
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.

Date:2014.11

Type:other

A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa

Creator:Jecz, Paulina | Bartosik, Aneta A | Glabski, Krzysztof | Jagura-Burdzy, Grazyna

Date:2015.03.20

Type:other

Binding and Spreading of ParB on DNA Determine Its Biological Function in Pseudomonas aeruginosa.

Creator:Kusiak, Magdalena | Gapczynska, Anna | Plochocka, Danuta | Thomas, Christopher M. | Jagura-Burdzy, Grazyna

Date:2011.07

Type:other

Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif

Creator:Kawalek, Adam | Bartosik, Aneta A | Glabski, Krzysztof | Jagura-Burdzy, Grazyna

Date:2018.04.10

Type:other

Identification of C-terminal hydrophobic residues important for dimerization and all known functions of ParB of Pseudomonas aeruginosa

Date:2012.01

Type:other

Transcriptional profiling of ParA and ParB mutants in actively dividing cells of an opportunistic human pathogen Pseudomonas aeruginosa.

Date:2014.01

Type:other

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

Creator:Kawalek, Adam | Wawrzyniak, Paweł | Bartosik, Aneta A | Jagura-Burdzy, Grazyna

Date:2020.01.11

Type:other

NudC Nudix hydrolase from Pseudomonas syringae, but not its counterpart from Pseudomonas aeruginosa, is a novel regulator of intracellular redox balance required for growth, motility and biofilm formation.

Creator:Modzelan, Marta | Kujawa, Martyna | Glabski, Krzysztof | Jagura-Burdzy, Grazyna | Kraszewska, Elzbieta

Date:2014.09

Type:other

Federacja Bibliotek Cyfrowych

Object's details: Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB.

Description

Similar objects