Description

• Title:
  Simple method for plating Escherichia coli bacteriophages forming very small plaques or no plaques under standard conditions
• Creator:
  Łoś, Joanna M;
  Golec, Piotr;
  Węgrzyn, Grzegorz;
  Węgrzyn, Alicja;
  Łoś, Marcin
• Description:
  The use of low concentrations (optimally 2.5 to 3.5 microg/ml, depending on top agar thickness) of ampicillin in the bottom agar of the plate allows for formation of highly visible plaques of bacteriophages which otherwise form extremely small plaques or no plaques on Escherichia coli lawns. Using this method, we were able to obtain plaques of newly isolated bacteriophages, propagated after induction of prophages present in six E. coli O157:H(-) strains which did not form plaques when standard plating procedures were employed.
• Object availability:
  free access
• Date:
  2008
• Type:
  Article;
  PeerReviewed
• Language:
  English;
  eng
• Format:
  application/pdf
• Publisher:
  American Society for Microbiology
• Relation: http://eprints.ibb.waw.pl/1039
• Subject:
  QH301 Biology;
  QH426 Genetics;
  QR Microbiology;
  QR355 Virology
• Identifier: http://eprints.ibb.waw.pl/1039/1/2008_0306-08.pdf ;
  Łoś, Joanna M and Golec, Piotr and Węgrzyn, Grzegorz and Węgrzyn, Alicja and Łoś, Marcin (2008) Simple method for plating Escherichia coli bacteriophages forming very small plaques or no plaques under standard conditions. Applied and Environmental Microbiology, 74 (16). pp. 5113-5120. ISSN 0099-2240
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other