Object's details: Coupling of transcription and replication machineries in the λ DNA replication initiation: evidence for direct interaction of Escherichia coli RNA polymerase and the lambda O protein

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Coupling of transcription and replication machineries in the λ DNA replication initiation: evidence for direct interaction of Escherichia coli RNA polymerase and the lambda O protein
Creator:
Szambowska, Anna;
Pierechod, Marcin;
Węgrzyn, Grzegorz;
Glinkowska, Monika
Description:
Transcription proceeding downstream of the lambda phage replication origin was previously shown to support initial steps of the lambda primosome assembly in vitro and to regulate frequency and directionality of lambda DNA replication in vivo. In this report, the data are presented indicating that the RNA polymerase beta subunit makes a direct contact with the lambdaO protein, a replication initiator of lambda phage. These results suggest that the role of RNA polymerase during the initiation of lambda phage DNA replication may be more complex than solely influencing DNA topology. Results demonstrated in this study also show that gyrase supercoiling activity stimulates the formation of a complex between lambdaO and RNA polymerase, suggesting that the introduction of negative supercoils by DNA gyrase, besides lowering the energy required for DNA strand separation, may play an additional role in modeling protein–protein interactions at early steps of DNA replication initiation.
Object availability:
free access
Date:
2011
Type:
Article;
PeerReviewed
Language:
English;
eng
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/258
Subject:
QH301 Biology
Identifier: http://eprints.ibb.waw.pl/258/1/NAR_2011.pdf ;
Szambowska, Anna and Pierechod, Marcin and Węgrzyn, Grzegorz and Glinkowska, Monika (2011) Coupling of transcription and replication machineries in the λ DNA replication initiation: evidence for direct interaction of Escherichia coli RNA polymerase and the lambda O protein. Nucleic Acids Research , 39 . pp. 168-177.
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Coupling of transcription and replication machineries in the λ DNA replication initiation: evidence for direct interaction of Escherichia coli RNA polymerase and the lambda O protein

Description

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