Description

• Title:
  PeptidePicker: A scientific workflow with web interface for selecting appropriate peptides for targeted proteomics experiments.
• Creator:
  Mohammed, Yassene;
  Domanski, Dominik;
  Jackson, Angela M;
  Smith, Derek S;
  Deelder, André M;
  Palmblad, Magnus;
  Borchers, Christoph H
• Description:
  One challenge in Multiple Reaction Monitoring (MRM)-based proteomics is to select the most appropriate surrogate peptides to represent a target protein. We present here a software package to automatically generate these most appropriate surrogate peptides for an LC/MRM-MS analysis. Our method integrates information about the proteins, their tryptic peptides, and the suitability of these peptides for MRM which is available online in UniProtKB, NCBI's dbSNP, ExPASy, PeptideAtlas, PRIDE, and GPMDB. The scoring algorithm reflects our knowledge in choosing the best candidate peptides for MRM, based on the uniqueness of the peptide in the targeted proteome, its physiochemical properties, and whether it previously has been observed. The modularity of the workflow allows further extension and additional selection criteria to be incorporated. We have developed a simple Web interface where the researcher provides the protein accession number, the subject organism, and peptide-specific options. Currently, the software is designed for human and mouse proteomes, but additional species can be easily be added. Our software improved the peptide selection by eliminating human error, considering multiple data sources and all of the isoforms of the protein, and resulted in faster peptide selection - approximately 50 proteins per hour compared to 8 per day.
• Object availability:
  free access
• Date:
  2014.04.22
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/msword
• Relation: http://eprints.ibb.waw.pl/713 ;http://www.sciencedirect.com/science/article/pii/S1874391914001857 ;DOI: 10.1016/j.jprot.2014.04.018
• Subject:
  Q Science (General)
• Identifier: http://eprints.ibb.waw.pl/713/7/PeptidePicker_v062_20131218_0044_ym2.docx ;
  Mohammed, Yassene and Domanski, Dominik and Jackson, Angela M and Smith, Derek S and Deelder, André M and Palmblad, Magnus and Borchers, Christoph H (2014) PeptidePicker: A scientific workflow with web interface for selecting appropriate peptides for targeted proteomics experiments. Journal of proteomics, 106C . pp. 151-161. ISSN 1876-7737
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other