Object's details: Cloning and characterization of the first member of the Nudix family from Arabidopsis thaliana.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Cloning and characterization of the first member of the Nudix family from Arabidopsis thaliana.
Creator:
Dobrzanska, Marta;
Szurmak, Blanka;
Wysłouch-Cieszyńska, Aleksandra;
Kraszewska, Elzbieta
Description:
The sequence motif commonly called a Nudix box, represented by (GX(5)EX(7)REVXEEXGU) is the marker of a widely distributed family of enzymes that catalyze the hydrolysis of a variety of nucleoside diphosphate derivatives. Here we describe the cloning and characterization of an Arabidopsis thaliana cDNA encoding a Nudix hydrolase that degrades NADH. The deduced amino acid sequence of AtNUDT1 contains 147 amino acids. The recombinant AtNUDT1 was expressed in Escherichia coli and purified. In the presence of Mn(2+) and the optimal pH of 7. 0, the recombinant AtNUDT1 catalyzed the hydrolysis of NADH with a K(m) value of 0. 36 mm. A V(max) of 12. 7 units mg (-1) for NADH was determined. The recombinant AtNUDT1 migrated as a dimer on a gel filtration column. Biochemical analysis of recombinant AtNUDT1 indicated that the first characterized member of the Nudix family from A. thaliana is a NADH pyrophosphatase.
Object availability:
free access
Date:
2002.12.27
Type:
Article;
PeerReviewed
Language:
English;
eng
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/292
Subject:
Q Science (General);
QH301 Biology
Identifier: http://eprints.ibb.waw.pl/292/1/50482.full.pdf ;
Dobrzanska, Marta and Szurmak, Blanka and Wysłouch-Cieszyńska, Aleksandra and Kraszewska, Elzbieta (2002) Cloning and characterization of the first member of the Nudix family from Arabidopsis thaliana. The Journal of biological chemistry, 277 (52). pp. 50482-6. ISSN 0021-9258
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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