Description

• Title:
  DNA damage and antioxidant properties of CORM-2 in normal and cancer cells
• Creator:
  Wysokiński, Daniel;
  Juszczak, Michał;
  Kluska, Magdalena;
  Woźniak, Katarzyna
• Contributor:
  Faculty of Biology and Environmental Protection, Department of Molecular Genetics, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland
• Description:
  In this study, we compared the effect of tricarbonyldichlororuthenium (II) dimer (CORM-2) and its CO-depleted molecule (iCORM-2) on human peripheral blood mononuclear cells (PBMCs) and human promyelocytic leukemia HL-60 cells. We determined cell viability, DNA damage and DNA repair kinetics. We also studied the effect of both compounds on DNA oxidative damage, free radical level and HO-1 gene expression. We showed that at low concentrations both CORM-2 and iCORM-2 stimulate PBMCs viability. After 24-h incubation, CORM-2 and iCORM-2, at the concentration of 100 µM, reduce the viability of both PBMCs and HL-60 cells. We also demonstrated that CORM-2 and iCORM-2, in the 0.01–100 µM concentration range, cause DNA damage such as strand breaks and alkaline labile sites. DNA damage was repaired efficiently only in HL-60 cells. CORM-2 significantly reduces oxidative stress induced by 1 mM H2O2 in normal and cancer cells. On the contrary, iCORM-2 in HL-60 cells increases the level of free radicals in the presence of 1 and 5 mM H2O2. We also revealed that both CORM-2 and iCORM-2 induce HO-1 gene expression. However, CORM-2 induces this gene to a greater extent than iCORM-2, especially in HL-60 cells at 100 µM. Finally, we showed that CORM-2 and iCORM-2 reduce H2O2-induced DNA oxidative damage. Furthermore, CORM-2 proved to be a compound with stronger antioxidant properties than iCORM-2. Our results suggest that both active CORM-2 and inactive iCORM-2 exert biological effects such as cyto- and genotoxicity, antioxidant properties and the ability to induce the HO-1 gene. The released CO as well as iCORM-2 can be responsible for these effects.
• Object availability:
  free access
• Rights:
  Uznanie autorstwa 4.0 Międzynarodowe;
  http://creativecommons.org/licenses/by/4.0
• Date:
  2021.08.31;
  2020
• Type:
  Article
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Springer Nature
• Relation:
  Scientific Reports;10;
  12200
• Subject:
  biochemistry;
  cancer;
  cell biology;
  molecular biology;
  molecular medicine
• Identifier:
  Juszczak, M., Kluska, M., Wysokiński, D. et al. DNA damage and antioxidant properties of CORM-2 in normal and cancer cells. Sci Rep 10, 12200 (2020). https://doi.org/10.1038/s41598-020-68948-6;
  http://hdl.handle.net/11089/38964 ;
  2045-2322;
  10.1038/s41598-020-68948-6
• Data provider:
  Repozytorium Uniwersytetu Łódzkiego
• Can I use it?:
  check license
• Type:
  image