Description

• Title:
  Experimental Relocation of the Mitochondrial ATP9 Gene to the Nucleus Reveals Forces Underlying Mitochondrial Genome Evolution
• Creator:
  Bietenhader, Mailis;
  di Rago, Jean-Paul;
  Martos, Alexandre;
  Tetaud, Emmanuel;
  Aiyar, Raeka S;
  Sellem, Carole H;
  Kucharczyk, Roza;
  Clauder-Münster, Sandra;
  Giraud, Marie-France;
  Godard, Franc¸ois;
  Salin, Bénédicte;
  Sagot, Isabelle;
  Gagneur, Julien;
  Dequard-Chablat, Michelle;
  Contamine, Veronique;
  Hermann-Le Denmat, Sylvie;
  Sainsard-Chanet, Annie;
  Steinmetz, Lars M.
• Description:
  Only a few genes remain in the mitochondrial genome retained by every eukaryotic organism that carry out essential functions and are implicated in severe diseases. Experimentally relocating these few genes to the nucleus therefore has both therapeutic and evolutionary implications. Numerous unproductive attempts have been made to do so, with a total of only 5 successes across all organisms. We have taken a novel approach to relocating mitochondrial genes that utilizes naturally nuclear versions from other organisms. We demonstrate this approach on subunit 9/c of ATP synthase, successfully relocating this gene for the first time in any organism by expressing the ATP9 genes from Podospora anserina in Saccharomyces cerevisiae. This study substantiates the role of protein structure in mitochondrial gene transfer: expression of chimeric constructs reveals that the P. anserina proteins can be correctly imported into mitochondria due to reduced hydrophobicity of the first transmembrane segment. Nuclear expression of ATP9, while permitting almost fully functional oxidative phosphorylation, perturbs many cellular properties, including cellular morphology, and activates the heat shock response. Altogether, our study establishes a novel strategy for allotopic expression of mitochondrial genes, demonstrates the complex adaptations required to relocate ATP9, and indicates a reason that this gene was only transferred to the nucleus during the evolution of multicellular organisms.
• Object availability:
  free access
• Date:
  2012.08
• Type:
  Article;
  PeerReviewed
• Language:
  English;
  eng
• Format:
  application/pdf
• Relation: http://eprints.ibb.waw.pl/420 ;http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420929
• Subject:
  QE Geology;
  QH426 Genetics;
  QK Botany;
  QL Zoology
• Identifier: http://eprints.ibb.waw.pl/420/1/PlosGenet2010OpenAcces.pdf ;
  Bietenhader, Mailis and di Rago, Jean-Paul and Martos, Alexandre and Tetaud, Emmanuel and Aiyar, Raeka S and Sellem, Carole H and Kucharczyk, Roza and Clauder-Münster, Sandra and Giraud, Marie-France and Godard, Franc¸ois and Salin, Bénédicte and Sagot, Isabelle and Gagneur, Julien and Dequard-Chablat, Michelle and Contamine, Veronique and Hermann-Le Denmat, Sylvie and Sainsard-Chanet, Annie and Steinmetz, Lars M. (2012) Experimental Relocation of the Mitochondrial ATP9 Gene to the Nucleus Reveals Forces Underlying Mitochondrial Genome Evolution. PLoS genetics, 8 (8). e1002876. ISSN 1553-7404
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other