Object's details: Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation
Creator:
Lichocka, Małgorzata;
Rymaszewski, Wojciech;
Morgiewicz, Karolina;
Barymow-Filoniuk, Izabela;
Chlebowski, Aleksander;
Sobczak, Mirosław;
Samuel, Marcus A;
Schmelzer, E.;
Krzymowska, Magdalena;
Hennig, Jacek
Description:
Background Pollen development is a strictly controlled post-meiotic process during which microspores differentiate into microgametophytes and profound structural and functional changes occur in organelles. Annexin 5 is a calcium- and lipid-binding protein that is highly expressed in pollen grains and regulates pollen development and physiology. To gain further insights into the role of ANN5 in Arabidopsis development, we performed detailed phenotypic characterization of Arabidopsis plants with modified ANN5 levels. In addition, interaction partners and subcellular localization of ANN5 were analyzed to investigate potential functions of ANN5 at cellular level. Results Here, we report that RNAi-mediated suppression of ANN5 results in formation of smaller pollen grains, enhanced pollen lethality, and delayed pollen tube growth. ANN5 RNAi knockdown plants also displayed aberrant development during the transition from the vegetative to generative phase and during embryogenesis, reflected by delayed bolting time and reduced embryo size, respectively. At the subcellular level, ANN5 was delivered to the nucleus, nucleolus, and cytoplasm, and was frequently localized in plastid nucleoids, suggesting a likely role in interorganellar communication. Furthermore, ANN5-YFP co-immunoprecipitated with RABE1b, a putative GTPase, and interaction in planta was confirmed in plastidial nucleoids using FLIM-FRET analysis. Conclusions Our findings let us to propose that ANN5 influences basal cell homeostasis via modulation of plastid activity during pollen maturation. We hypothesize that the role of ANN5 is to orchestrate the plastidial and nuclear genome activities via protein-protein interactions however not only in maturing pollen but also during the transition from the vegetative to the generative growth and seed development. Keywords
Object availability:
free access
Date:
2018.05
Type:
Article;
PeerReviewed
Language:
English
Format:
text/html
Relation: http://eprints.ibb.waw.pl/1570 ;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127919/
Subject:
QK Botany;
QP Physiology
Identifier: http://eprints.ibb.waw.pl/1570/2/10.1186/s12870-018-1405-3 ;
Lichocka, Małgorzata and Rymaszewski, Wojciech and Morgiewicz, Karolina and Barymow-Filoniuk, Izabela and Chlebowski, Aleksander and Sobczak, Mirosław and Samuel, Marcus A and Schmelzer, E. and Krzymowska, Magdalena and Hennig, Jacek (2018) Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation. BMC Plant Biology, 18 (183).
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

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