Description

• Title:
  DnaA-stimulated transcriptional activation of orilambda: Escherichia coli RNA polymerase beta subunit as a transcriptional activator contact site
• Creator:
  Szalewska-Pałasz, Agnieszka;
  Węgrzyn, Alicja;
  Błaszczak, Adam;
  Taylor, Karol;
  Węgrzyn, Grzegorz
• Description:
  We present evidence that Escherichia coli RNA polymerase beta subunit may be a transcriptional activator contact site. Stimulation of the activity of the pR promoter by DnaA protein is necessary for replication of plasmids derived from bacteriophage lambda. We found that DnaA activates the pR promoter in vitro. Particular mutations in the rpoB gene were able to suppress negative effects that certain dnaA mutations had on the replication of lambda plasmids; this suppression was allele-specific. When a potential DnaA-binding sequence located several base pairs downstream of the pR promoter was scrambled by in vitro mutagenesis, the pR promoter was no longer activated by DnaA both in vivo and in vitro. Therefore, we conclude that DnaA may contact the beta subunit of RNA polymerase during activation of the pR promoter. A new classification of prokaryotic transcriptional activators is proposed.
• Object availability:
  free access
• Date:
  1998
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  National Academy of Sciences
• Relation: http://eprints.ibb.waw.pl/1037 ;http://www.pnas.org/cgi/pmidlookup?view=long&pmid=9539721 ;
  PMID: 9539721
• Subject:
  QH301 Biology;
  QH426 Genetics;
  QR Microbiology
• Identifier: http://eprints.ibb.waw.pl/1037/1/1998_pq004241.pdf ;
  Szalewska-Pałasz, Agnieszka and Węgrzyn, Alicja and Błaszczak, Adam and Taylor, Karol and Węgrzyn, Grzegorz (1998) DnaA-stimulated transcriptional activation of orilambda: Escherichia coli RNA polymerase beta subunit as a transcriptional activator contact site. Proceedings of the National Academy of Sciences of the United States of America, 95 (8). pp. 4241-4246. ISSN 1091-6490
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other