Description

• Title:
  Activity and structure of EcoKMcrA.
• Creator:
  Czapinska, Honorata;
  Kowalska, Monika;
  Zagorskaitė, Evelina;
  Manakova, Elena;
  Slyvka, Anton;
  Xu, Shuang-yong;
  Siksnys, Virginijus;
  Sasnauskas, Giedrius;
  Bochtler, Matthias
• Description:
  Escherichia coli McrA (EcoKMcrA) acts as a methylcytosine and hydroxymethylcytosine dependent restriction endonuclease. We present a biochemical characterization of EcoKMcrA that includes the first demonstration of its endonuclease activity, small angle X-ray scattering (SAXS) data, and a crystal structure of the enzyme in the absence of DNA. Our data indicate that EcoKMcrA dimerizes via the anticipated C-terminal HNH domains, which together form a single DNA binding site. The N-terminal domains are not homologous to SRA domains, do not interact with each other, and have separate DNA binding sites. Electrophoretic mobility shift assay (EMSA) and footprinting experiments suggest that the N-terminal domains can sense the presence and sequence context of modified cytosines. Pyrrolocytosine fluorescence data indicate no base flipping. In vitro, EcoKMcrA DNA endonuclease activity requires Mn2+ ions, is not strictly methyl dependent, and is not observed when active site variants of the enzyme are used. In cells, EcoKMcrA specifically restricts DNA that is modified in the correct sequence context. This activity is impaired by mutations of the nuclease active site, unless the enzyme is highly overexpressed.
• Object availability:
  free access
• Date:
  2018.10.12
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  Oxford University Press
• Relation: http://eprints.ibb.waw.pl/1785 ;https://academic.oup.com/nar/article/46/18/9829/5068907 ;doi: 10.1093/nar/gky731
• Subject:
  Q Science (General)
• Identifier: http://eprints.ibb.waw.pl/1785/1/2018_czapinska.pdf ;
  Czapinska, Honorata and Kowalska, Monika and Zagorskaitė, Evelina and Manakova, Elena and Slyvka, Anton and Xu, Shuang-yong and Siksnys, Virginijus and Sasnauskas, Giedrius and Bochtler, Matthias (2018) Activity and structure of EcoKMcrA. Nucleic Acids Research, 46 (18). pp. 9829-9841. ISSN 0305-1048
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other