Object's details: New redox-active layer create via epoxy-amine reaction - The base of genosensor for the detection of specific DNA and RNA sequences of avian influenza virus H5N1.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
New redox-active layer create via epoxy-amine reaction - The base of genosensor for the detection of specific DNA and RNA sequences of avian influenza virus H5N1.
Creator:
Malecka, Kamila;
Stachyra, Anna;
Góra-Sochacka, Anna;
Sirko, Agnieszka;
Zagórski-Ostoja, Włodzimierz;
Dehaen, Wim;
Radecka, Hanna;
Radecki, Jerzy
Description:
This paper concerns the development of a redox-active monolayer and its application for the construction of an electrochemical genosensor designed for the detection of specific DNA and RNA oligonucleotide sequences related to the avian influenza virus (AIV) type H5N1. This new redox layer was created on a gold electrode surface step by step. Cyclic Voltammetry, Osteryoung Square-Wave Voltammetry and Differential Pulse Voltammetry were used for its characterization. This new redox-active layer was applied for the construction of the DNA biosensor. The NH2-NC3 probe (20-mer) was covalently attached to the gold electrode surface via a "click" reaction between the amine and an epoxide group. The hybridization process was monitored using the Osteryoung Square-Wave Voltammetry. The 20-mer DNA and ca. 280-mer RNA oligonucleotides were used as the targets. The constructed genosensor was capable to determine complementary oligonucleotide sequences with a detection limit in the pM range. It is able to distinguish the different position of the part RNA complementary to the DNA probe. The genosensor was very selective. The 20-mer DNA as well as the 280-mer RNA oligonucleotides without a complementary sequence generated a weak signal.
Object availability:
free access
Date:
2015.11.04
Type:
Article;
PeerReviewed
Language:
English;
eng
Format:
application/pdf
Publisher:
Elsevier
Relation: http://eprints.ibb.waw.pl/956 ;http://www.sciencedirect.com/science/article/pii/S0956566314008689 ;doi: 10.1016/j.bios.2014.10.069.
Subject:
QD Chemistry;
QR Microbiology;
QR355 Virology
Identifier: http://eprints.ibb.waw.pl/956/1/1-s2.0-S0956566314008689-main.pdf ;
Malecka, Kamila and Stachyra, Anna and Góra-Sochacka, Anna and Sirko, Agnieszka and Zagórski-Ostoja, Włodzimierz and Dehaen, Wim and Radecka, Hanna and Radecki, Jerzy (2015) New redox-active layer create via epoxy-amine reaction - The base of genosensor for the detection of specific DNA and RNA sequences of avian influenza virus H5N1. Biosensors & Bioelectronics, 65C . pp. 427-434. ISSN 1873-4235
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: New redox-active layer create via epoxy-amine reaction - The base of genosensor for the detection of specific DNA and RNA sequences of avian influenza virus H5N1.

Description

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