Object's details: Catalytic activities of Werner protein are affected by adduction with 4-hydroxy-2-nonenal

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Catalytic activities of Werner protein are affected by adduction with 4-hydroxy-2-nonenal
Creator:
Czerwińska, Jolanta;
Poznański, Jarosław;
Dębski, Janusz;
Bukowy, Zuzanna;
Bohr, Vilhelm A;
Tudek, Barbara;
Speina, Elzbieta
Description:
4-Hydroxy-2-nonenal (HNE) is a reactive �,�- unsaturated aldehyde generated during oxidative stress and subsequent peroxidation of polyunsaturated fatty acids. Here, Werner protein (WRN) was identified as a novel target for modification by HNE. Werner syndrome arises through mutations in the WRN gene that encodes the RecQ DNA helicase which is critical for maintaining genomic stability. This hereditary disease is associated with chromosomal instability, premature aging and cancer predisposition. WRN appears to participate in the cellular response to oxidative stress and cells devoid of WRN display elevated levels of oxidative DNA damage. We demonstrated that helicase/ATPase and exonuclease activities of HNE-modified WRN protein were inhibited both in vitro and in immunocomplexes purified from the cell extracts. Sites of HNE adduction in human WRN were identified at Lys577, Cys727, His1290, Cys1367, Lys1371 and Lys1389. We applied in silico modeling of the helicase and RQC domains of WRN protein with HNE adducted to Lys577 and Cys727 and provided a potential mechanism of the observed deregulation of the protein catalytic activities. In light of the obtained results, we postulate that HNE adduction to WRN is a post-translational modification, which may affect WRN conformational stability and function, contributing to features and diseases associated with premature senescence.
Object availability:
free access
Date:
2014.08.28
Type:
Article;
PeerReviewed
Language:
eng
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/746 ;http://nar.oxfordjournals.org/content/early/2014/08/28/nar.gku783.full ;
25170083
Subject:
Q Science (General)
Identifier: http://eprints.ibb.waw.pl/746/1/nar.gku783.full.pdf ;http://eprints.ibb.waw.pl/746/5/Supplementary_Material.pdf ;
Czerwińska, Jolanta and Poznański, Jarosław and Dębski, Janusz and Bukowy, Zuzanna and Bohr, Vilhelm A and Tudek, Barbara and Speina, Elzbieta (2014) Catalytic activities of Werner protein are affected by adduction with 4-hydroxy-2-nonenal. Nucleic Acids Research, 43 (17). pp. 11119-11135.
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

Catalytic activities of Werner protein are affected by adduction with 4-hydroxy-2-nonenal

Date:2014.08.28

Type:other

8-Oxoguanine incision activity is impaired in lung tissues of NSCLC patients with the polymorphism of OGG1 and XRCC1 genes

Date:2011

Type:other

Human RECQL5beta stimulates flap endonuclease 1.

Date:2010

Type:other

Contribution of a halogen bond to binding of halogenated ligands by protein kinases (Rola wiązania halogenowego w oddziaływaniu halogenowanych ligandów z kinazami białkowymi)

Creator:Poznański, Jarosław

Date:2015

Type:other

Synthesis of Novel Halogenated Heterocycles Based on o-Phenylenediamine and Their Interactions with the Catalytic Subunit of Protein Kinase CK2

Creator:Winiewska-Szajewska, Maria | Maciejewska, Agnieszka Monika | Speina, Elzbieta | Poznański, Jarosław | Paprocki, Daniel

Date:2021

Type:other

Halogen bonds involved in binding of halogenated ligands by protein kinases.

Creator:Poznański, Jarosław | Winiewska, Maria | Czapinska, Honorata | Poznańska, Anna | Shugar, David

Date:2016

Type:other

Thermodynamics parameters for binding of halogenated benzotriazole inhibitors of human protein kinase CK2α.

Creator:Winiewska, Maria | Kucińska, Katarzyna | Makowska, Małgorzata | Poznański, Jarosław | Shugar, David

Date:2015.10

Type:other

Proteins contribute insignificantly to the intrinsic buffering capacity of yeast cytoplasm.

Creator:Poznański, Jarosław | Szczesny, Pawel | Ruszczyńska-Bartnik, Katarzyna | Zielenkiewicz, Piotr | Paczek, Leszek

Date:2013.12.01

Type:other

Federacja Bibliotek Cyfrowych

Object's details: Catalytic activities of Werner protein are affected by adduction with 4-hydroxy-2-nonenal

Description

Similar objects