Description

• Title:
  Using Multiplex-Staining to Study Changes in the Maize Leaf Phosphoproteome in Response to Mechanical Wounding
• Creator:
  Lewandowska-Gnatowska, Elżbieta;
  Johnston, M L;
  Antoine, W;
  Szczegielniak, Jadwiga;
  Muszyńska, Grażyna;
  Miernyk, J A
• Description:
  Mechanical wounding of 2-week-old maize (Zea mays L.) leaves, one of the first steps in both pathogen infection and herbivore attack, stimulates metabolism and activates signal transduction pathways dedicated to defense and recovery. The signaling pathways include reversible protein phosphorylation which can modulate protein activities, and transmit signals within cellular pathways and networks. We have used multiplex-staining of high-resolution 2D gels for protein (Sypro Ruby) and phosphorylation (Pro-Q Diamond) as a strategy for quantifying changes in the stoichiometry of phosphorylation after wounding for 270 protein spots. Rigorous statistical analysis of the time-index data allowed us to accept patterns of change in 125 of the spots as non-random, and these patterns were assigned to five clusters. A reliable identity was assigned to 21 selected proteins, most of which have been previously described as phospho-proteins. The results suggest that analysis of protein spots from high-resolution 2D gels by multiplex-staining for protein plus phosphorylation is a strategy that can be broadly useful for study of how the phospho-proteome responds to abiotic stress.
• Object availability:
  free access
• Date:
  2011.07
• Type:
  Article;
  NonPeerReviewed
• Language:
  English
• Format:
  application/msword
• Publisher:
  Elsevier
• Relation: http://eprints.ibb.waw.pl/159 ;doi:10.1016/j.phytochem.2011.01.030. ;
  10.1016/j.phytochem.2011.01.030
• Subject:
  QP Physiology;
  SB Plant culture
• Identifier: http://eprints.ibb.waw.pl/159/1/Manuscript.doc ;
  Lewandowska-Gnatowska, Elżbieta and Johnston, M L and Antoine, W and Szczegielniak, Jadwiga and Muszyńska, Grażyna and Miernyk, J A (2011) Using Multiplex-Staining to Study Changes in the Maize Leaf Phosphoproteome in Response to Mechanical Wounding. Phytochemistry, 72 (10). pp. 1285-1292. ISSN 0031-9422
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  image