Opis

• Tytuł:
  Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry
• Twórca:
  Ahsan, Nagib;
  Kataya, Amr R. A.;
  Rao, Shyama Prasad;
  Swatek, Kirby N.;
  Rashaun S., Wilson;
  Louis J., Meyer;
  Alejandro, Tovar-Mendez;
  Severin, Stevenson;
  Justyna, Maszkowska;
  Grazyna, Dobrowolska;
  Qiuming, Yao;
  Dong, Xu;
  Jay J, Thelen
• Opis:
  Members of the calcium-dependent protein kinase (CDPK/CPK) and SNF-related protein kinase (SnRK) superfamilies are commonly found in plants and some protists. Our knowledge of client specificity of the members of this superfamily is fragmentary. As this family is represented by over 30 members in Arabidopsis thaliana, the identification of kinase-specific and overlapping client relationships is crucial to our understanding the nuances of this large family of kinases as directed towards signal transduction pathways. Herein, we used the kinase client (KiC) assay—a relative, quantitative, high-throughput mass spectrometry-based in vitro phosphorylation assay—to identify and characterize potential CPK/SnRK targets of Arabidopsis. Eight CPKs (1, 3, 6, 8, 17, 24, 28, and 32), four SnRKs (subclass 1 and 2), and PPCK1 and PPCK2 were screened against a synthetic peptide library that contains 2095 peptides and 2661 known phosphorylation sites. A total of 625 in vitro phosphorylation sites corresponding to 203 non-redundant proteins were identified. The most promiscuous kinase, CPK17, had 105 candidate target proteins, many of which had already been discovered. Sequence analysis of the identified phosphopeptides revealed four motifs: LxRxxS, RxxSxxR, RxxS, and LxxxxS, that were significantly enriched among CPK/SnRK clients. The results provide insight into both CPK- and SnRK-specific and overlapping signaling network architectures and recapitulate many known in vivo relationships validating this large-scale approach towards discovering kinase targets.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2024.05.27
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski
• Format:
  application/pdf
• Wydawca:
  MDPI
• Relacja: http://eprints.ibb.waw.pl/2432 ;https://www.mdpi.com/2223-7747/13/11/1481 ;doi: 10.3390/plants13111481.
• Temat i słowa kluczowe:
  QK Botany
• Identyfikator: http://eprints.ibb.waw.pl/2432/1/plants-13-01481.pdf ;
  Ahsan, Nagib and Kataya, Amr R. A. and Rao, Shyama Prasad and Swatek, Kirby N. and Rashaun S., Wilson and Louis J., Meyer and Alejandro, Tovar-Mendez and Severin, Stevenson and Justyna, Maszkowska and Grazyna, Dobrowolska and Qiuming, Yao and Dong, Xu and Jay J, Thelen (2024) Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry. Plants, 13 (11). p. 1481.
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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