Opis

• Tytuł:
  Is DNA methylation modulated by wounding-induced oxidative burst in maize?
• Twórca:
  Lewandowska-Gnatowska, Elżbieta;
  Polkowska-Kowalczyk, Lidia;
  Szczegielniak, Jadwiga;
  Barciszewska, Mirosława;
  Barciszewski, Jan;
  Muszyńska, Grażyna
• Opis:
  Plants respond to environmental changes by modifying gene expression. One of the mechanisms regulating gene expression is methylation of cytosine to 5-methylcytosine (m5C) which modulates gene expression by changing chromatin structure. Methylation/demethylation processes affect genes that are controlled upon environmental stresses. Here, on account of the regulatory role of m5C, we evaluate the content of m5C in DNA from normal and wound-damaged maize leaves. Wounding leads to a transient decrease of the global DNA methylation level ca 20-30% 1 hour after the treatment followed by a return to the initial level within the next hours. Similar results were obtained using of radio-labelled nucleotides separated by Thin Layer Chromatography (TLC) or using m5C-specific Enzyme-Linked Immunosorbent Assay (ELISA). Wounding induced in maize leaves a two-step oxidative stress, an early one just after wounding and the second two hours later. It coincides with the transient changes of the cytosine methylation level. In the stress-inducible maize calcium-dependent protein kinase ZmCPK11 gene wounding transiently reduced methylation of cytosines 100 and 126 in the first exon.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2014.09
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski
• Format:
  application/pdf
• Wydawca:
  Elsevier Masson SAS
• Relacja: http://eprints.ibb.waw.pl/720 ;http://dx.doi.org/10.1016/j.plaphy.2014.06.003 ;DOI: 10.1016/j.plaphy.2014.06.003
• Temat i słowa kluczowe:
  QP Physiology
• Identyfikator: http://eprints.ibb.waw.pl/720/1/Manuskrypt_29_05_2014r.pdf ;
  Lewandowska-Gnatowska, Elżbieta and Polkowska-Kowalczyk, Lidia and Szczegielniak, Jadwiga and Barciszewska, Mirosława and Barciszewski, Jan and Muszyńska, Grażyna (2014) Is DNA methylation modulated by wounding-induced oxidative burst in maize? Plant Physiology and Biochemistry, 82 . pp. 202-208. ISSN 0981-9428 (In Press)
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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