Szczegóły obiektu: Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Tytuł:
Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.
Twórca:
Kruszewska, Joanna S.;
Gorka-Niec, Wioletta;
Kania, Anna;
Perlinska-Lenart, Urszula;
Smolenska-Sym, Gabriela;
Palamarczyk, Grazyna
Opis:
In fungi, transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases. Integration of additional copies of the pmt1 gene into Trichoderma reesei genome unexpectedly resulted in the silencing of pmt1 expression. Strains carrying the additional copies of pmt1 gene exhibited lower total activity of protein O-mannosyltransferases, lower O- and N-glycosylation of secreted proteins and showed defects in their cell wall composition. Moreover, the strains grew slowly on solid medium and were hypersensitive to an antifungal reagent, Calcofluor white. These results indicate that protein O-manosyltransferases are required for proper cell wall formation, and their decreased activity influences not only O- but also N-glycosylation.
Dostępność obiektu:
Dostęp otwarty
Data:
2011
Typ:
Article;
PeerReviewed
Język:
angielski
Format:
application/msword
Relacja: http://eprints.ibb.waw.pl/149 ;http://www.sciencedirect.com/science/article/pii/S1878614610001820
Temat i słowa kluczowe:
Q Science (General)
Identyfikator: http://eprints.ibb.waw.pl/149/1/PMTI_FB-R.doc ;
Kruszewska, Joanna S. and Gorka-Niec, Wioletta and Kania, Anna and Perlinska-Lenart, Urszula and Smolenska-Sym, Gabriela and Palamarczyk, Grazyna (2011) Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition. Fungal Biology, 115 . pp. 124-132.
Dostawca danych:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Szczegóły obiektu: Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.

Opis

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