Object's details: Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.
Creator:
Kruszewska, Joanna S.;
Gorka-Niec, Wioletta;
Kania, Anna;
Perlinska-Lenart, Urszula;
Smolenska-Sym, Gabriela;
Palamarczyk, Grazyna
Description:
In fungi, transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases. Integration of additional copies of the pmt1 gene into Trichoderma reesei genome unexpectedly resulted in the silencing of pmt1 expression. Strains carrying the additional copies of pmt1 gene exhibited lower total activity of protein O-mannosyltransferases, lower O- and N-glycosylation of secreted proteins and showed defects in their cell wall composition. Moreover, the strains grew slowly on solid medium and were hypersensitive to an antifungal reagent, Calcofluor white. These results indicate that protein O-manosyltransferases are required for proper cell wall formation, and their decreased activity influences not only O- but also N-glycosylation.
Object availability:
free access
Date:
2011
Type:
Article;
PeerReviewed
Language:
English
Format:
application/msword
Relation: http://eprints.ibb.waw.pl/149 ;http://www.sciencedirect.com/science/article/pii/S1878614610001820
Subject:
Q Science (General)
Identifier: http://eprints.ibb.waw.pl/149/1/PMTI_FB-R.doc ;
Kruszewska, Joanna S. and Gorka-Niec, Wioletta and Kania, Anna and Perlinska-Lenart, Urszula and Smolenska-Sym, Gabriela and Palamarczyk, Grazyna (2011) Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition. Fungal Biology, 115 . pp. 124-132.
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Federacja Bibliotek Cyfrowych

Object's details: Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.

Description

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