Object's details: Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis
Creator:
Dziedzic, Dariusz;
Narajczyk, Magdalena;
Gabig-Cimińska, Magdalena;
Jakóbkiewicz-Banecka, Joanna
Description:
It has been demonstrated recently that it is possible to decrease expression of genes coding for enzymes involved in synthesis of glycosaminoglycans (GAGs) by using specific siRNAs which interfere with stability of particular mRNAs. This procedure has been proposed as a potential treatment for patients suffering from mucopolysaccharidoses, a group of inherited metabolic diseases caused by dysfunction of enzymes required for GAG degradation, and resultant storage of these compounds in cells of affected persons. Here, we asked if the simultaneous use two species of specific siRNAs aimed at silencing two genes involved in particular steps of GAG synthesis may be more effective than the use of single siRNA. We found that inhibition of GAG synthesis in cells treated with two siRNAs is generally more effective than using single siRNAs. However, the differences were not statistically significant, therefore the potential benefit from the use of two siRNAs over the use of a single siRNA is doubtful in the light of the cost-benefit ratio and possibly stronger side-effects of the putative therapy.
Object availability:
free access
Date:
2012
Type:
Article;
PeerReviewed
Language:
English
Format:
application/msword
Relation: http://eprints.ibb.waw.pl/385
Subject:
Q Science (General);
QH426 Genetics
Identifier: http://eprints.ibb.waw.pl/385/1/pap_siRNA_2-9popr.docx ;
Dziedzic, Dariusz and Narajczyk, Magdalena and Gabig-Cimińska, Magdalena and Jakóbkiewicz-Banecka, Joanna (2012) Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis. Acta Biochimica Polonica, 59 (2). pp. 293-298.
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis

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