Description

• Title:
  Gly-His-Thr-Asp-Amide, an Insulin-Activating Peptide from the Human Pancreas Is a Strong Cu(II) but a Weak Zn(II) Chelator
• Creator:
  Kotuniak, Radosław;
  Frączyk, Tomasz;
  Skrobecki, Piotr;
  Płonka, Dawid;
  Bal, Wojciech
• Description:
  The Cu(II) and Zn(II) binding abilities of Gly-His-Thr-Asp-amide (GHTD-am), a tetrapeptide coreleased from the pancreas along with insulin, were studied using UV-vis and circular dichroism spectroscopies, potentiometry, and calorimetry. GHTD-am is a very strong Cu(II) chelator, forming a three-nitrogen complex with a conditional affinity constant C K at pH 7.4 of 4.5 × 10^12 M^-1. The fourth coordination site can be occupied by a solvent molecule or a ternary ligand, such as imidazole, with C K on the order of several hundred reciprocal molar. The Zn(II) binding ability of GHTD-am is relatively weak, with C K values at pH 7.4 of 3.0 × 10^4 and 2.0 × 10^3 M-1 for the first and second GHTD-am molecule coordinated, respectively. These results are discussed in light of the modes of interactions of Zn(II) and Cu(II) ions with insulin. A direct effect of GHTD-am on the Zn(II) interactions with insulin is unlikely, but its Cu(II) complex may have a biological relevance because of its high affinity and ability to form ternary complexes.
• Object availability:
  free access
• Date:
  2018
• Type:
  Article;
  PeerReviewed
• Publisher:
  ACS Publications
• Relation: http://eprints.ibb.waw.pl/1657 ;https://pubs.acs.org/doi/10.1021/acs.inorgchem.8b02841
• Subject:
  QD Chemistry;
  QH301 Biology
• Identifier:
  Kotuniak, Radosław and Frączyk, Tomasz and Skrobecki, Piotr and Płonka, Dawid and Bal, Wojciech (2018) Gly-His-Thr-Asp-Amide, an Insulin-Activating Peptide from the Human Pancreas Is a Strong Cu(II) but a Weak Zn(II) Chelator. Inorganic Chemistry . ISSN Print Edition ISSN: 0020-1669 Web Edition ISSN: 1520-510X (In Press)
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other