Description

• Title:
  Intermediate Cu(II)-Thiolate Species in the Reduction of Cu(II)GHK by Glutathione: A Handy Chelate for Biological Cu(II) Reduction
• Creator:
  Ufnalska, Iwona;
  Drew, Simon;
  Zhukov, Igor;
  Szutkowski, Kosma;
  Wawrzyniak, Urszula E.;
  Wróblewski, Wojciech;
  Frączyk, Tomasz;
  Bal, Wojciech
• Description:
  Gly-His-Lys (GHK) is a tripeptide present in the human bloodstream that exhibits a number of biological functions. Its activity is attributed to the copper-complexed form, Cu(II)GHK. Little is known, however, about the molecular aspects of the mechanism of its action. Here, we examined the reaction of Cu(II)GHK with reduced glutathione (GSH), which is the strongest reductant naturally occurring in human plasma. Spectroscopic techniques (UV–vis, CD, EPR, and NMR) and cyclic voltammetry helped unravel the reaction mechanism. The impact of temperature, GSH concentration, oxygen access, and the presence of ternary ligands on the reaction were explored. The transient GSH-Cu(II)GHK complex was found to be an important reaction intermediate. The kinetic and redox properties of this complex, including tuning of the reduction rate by ternary ligands, suggest that it may provide a missing link in copper trafficking as a precursor of Cu(I) ions, for example, for their acquisition by the CTR1 cellular copper transporter.
• Object availability:
  free access
• Date:
  2021
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Publisher:
  ACS Publications
• Relation: http://eprints.ibb.waw.pl/2113 ;https://doi.org/10.1021/acs.inorgchem.1c02669
• Subject:
  QD Chemistry;
  QH301 Biology
• Identifier: http://eprints.ibb.waw.pl/2113/1/acs.inorgchem.1c02669.pdf ;
  Ufnalska, Iwona and Drew, Simon and Zhukov, Igor and Szutkowski, Kosma and Wawrzyniak, Urszula E. and Wróblewski, Wojciech and Frączyk, Tomasz and Bal, Wojciech (2021) Intermediate Cu(II)-Thiolate Species in the Reduction of Cu(II)GHK by Glutathione: A Handy Chelate for Biological Cu(II) Reduction. Inorganic Chemistry, 60 (23). pp. 18048-18057. ISSN Print Edition ISSN: 0020-1669 Web Edition ISSN: 1520-510X
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other