Object's details: Arabidopsis DXO1 links RNA turnover and chloroplast function independently of its enzymatic activity

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Arabidopsis DXO1 links RNA turnover and chloroplast function independently of its enzymatic activity
Creator:
Kwasnik, Aleksandra;
Wang, Vivien Ya-Fan;
Krzyszton, Michal;
Gozdek, Agnieszka;
Zakrzewska-Placzek, Monika;
Stepniak, Karolina;
Poznański, Jarosław;
Tong, Liang;
Kufel, Joanna
Description:
The DXO family of proteins participates in eukaryotic mRNA 5'-end quality control, removal of non-canonical NAD+ cap and maturation of fungal rRNA precursors. In this work, we characterize the Arabidopsis thaliana DXO homolog, DXO1. We demonstrate that the plant-specific modification within the active site negatively affects 5'-end capping surveillance properties of DXO1, but has only a minor impact on its strong deNADding activity. Unexpectedly, catalytic activity does not contribute to striking morphological and molecular aberrations observed upon DXO1 knockout in plants, which include growth and pigmentation deficiency, global transcriptomic changes and accumulation of RNA quality control siRNAs. Conversely, these phenotypes depend on the plant-specific N-terminal extension of DXO1. Pale-green coloration of DXO1-deficient plants and our RNA-seq data reveal that DXO1 affects chloroplast-localized processes. We propose that DXO1 mediates the connection between RNA turnover and retrograde chloroplast-to-nucleus signaling independently of its deNADding properties.
Object availability:
free access
Date:
2019
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/1723 ;http://doi.org/10.1093/nar/gkz100 ;doi:10.1093/nar/gkz100
Subject:
Q Science (General)
Identifier: http://eprints.ibb.waw.pl/1723/1/jp2019_NAR_gkz100.pdf ;
Kwasnik, Aleksandra and Wang, Vivien Ya-Fan and Krzyszton, Michal and Gozdek, Agnieszka and Zakrzewska-Placzek, Monika and Stepniak, Karolina and Poznański, Jarosław and Tong, Liang and Kufel, Joanna (2019) Arabidopsis DXO1 links RNA turnover and chloroplast function independently of its enzymatic activity. Nucleic Acids Research, 47 (9). pp. 4751-4764. ISSN 0305-1048
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Arabidopsis DXO1 links RNA turnover and chloroplast function independently of its enzymatic activity

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