Description

• Title:
  Uridylation regulates mRNA decay directionality in fission yeast
• Creator:
  Grochowski, Maciej;
  Lipińska-Zubrycka, Lidia;
  Townsend, StJohn;
  Golisz-Mocydlarz, Anna;
  Zakrzewska-Płaczek, Monika;
  Brzyżek, Grzegorz;
  Jurković, Borna;
  Świeżewski, Szymon;
  Ralser, Markus;
  Małecki, Michał
• Description:
  Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5’ to 3’ or 3’ to 5’ direction. Pervasive terminal uridylation is implicated in mRNA degradation, however, its functional relevance for bulk mRNA turnover remains poorly understood. In this study, we employ genome-wide 3’-RACE (gw3’-RACE) in the model system fission yeast to elucidate the role of uridy- lation in mRNA turnover. We observe widespread uridylation of shortened poly(A) tails, promoting efficient 5’ to 3’ mRNA decay and ensuring timely and controlled mRNA degradation. Inhibition of this uridylation process leads to excessive deadenylation and enhanced 3’ to 5’ mRNA decay accompanied by oligouridylation. Strikingly we found that uridylation of poly(A) tails and oli- gouridylation of non-polyadenylated substrates are catalysed by different terminal uridyltransferases Cid1 and Cid16 respectively. Our study sheds new light on the intricate regulatory mechanisms underlying bulk mRNA turnover, demonstrating the role of uridylation in modulating mRNA decay pathways.
• Object availability:
  free access
• Date:
  2024
• Type:
  Article;
  PeerReviewed
• Language:
  English
• Format:
  application/pdf
• Relation: http://eprints.ibb.waw.pl/2458 ;http://doi.org/10.1038/s41467-024-50824-w ;doi:10.1038/s41467-024-50824-w
• Subject:
  Q Science (General)
• Identifier: http://eprints.ibb.waw.pl/2458/1/s41467-024-50824-w.pdf ;
  Grochowski, Maciej and Lipińska-Zubrycka, Lidia and Townsend, StJohn and Golisz-Mocydlarz, Anna and Zakrzewska-Płaczek, Monika and Brzyżek, Grzegorz and Jurković, Borna and Świeżewski, Szymon and Ralser, Markus and Małecki, Michał (2024) Uridylation regulates mRNA decay directionality in fission yeast. Nature Communications, 15 (1). ISSN 2041-1723
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other