Object's details: Recombinant Mouse GM-CSF is glycosylated in transgenic tobacco and maintains its biological activity

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Recombinant Mouse GM-CSF is glycosylated in transgenic tobacco and maintains its biological activity
Creator:
Góra-Sochacka, Anna;
Redkiewicz, Patrycja;
Napiórkowska, Bogusława;
Gaganidze, Dali;
Brodzik, Robert;
Sirko, Agnieszka
Description:
The granulocyte–macrophage colony-stimulating factor (GM-CSF) is a cytokine with many important applications and, due to its immunostimulatory properties, could also be used as a vaccine adjuvant. A simple strategy to produce recombinant mouse GM-CSF (mGM-CSF) in transgenic Nicotiana tabacum plants was used in this study. The mGM-CSF cDNA followed by the sequence encoding endoplasmic reticulum retention signal (KDEL) was cloned into the ImpactVector under the control of the strong promoter from the gene encoding a small subunit of Rubisco. In transgenic plants the accumulation level of recombinant mGM-CSF varied in the individual transformants from 8 to 19 μg/g of fresh leaf tissue, which makes up to 0.3% of total soluble protein. In most analyzed plants, the apparent molecular weight of the recombinant protein was larger than predicted due to its N-glycosylation, presumably in 2 sites. The recombinant plant-produced murine GM-CSF retained its biological activity as confi rmed in vitro in proliferation assay using a mouse cell line, which is growth-dependent on GM-CSF.
Object availability:
free access
Date:
2009.12.28
Type:
Article;
PeerReviewed
Publisher:
Mary Ann Liebert, Inc
Relation: http://eprints.ibb.waw.pl/6 ;DOI: 10.1089/jir.2009.0053
Subject:
QK Botany;
RM Therapeutics. Pharmacology
Identifier:
Góra-Sochacka, Anna and Redkiewicz, Patrycja and Napiórkowska, Bogusława and Gaganidze, Dali and Brodzik, Robert and Sirko, Agnieszka (2009) Recombinant Mouse GM-CSF is glycosylated in transgenic tobacco and maintains its biological activity. Journal of Interferon and Cytokine Research, 30 (3).
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Recombinant Mouse GM-CSF is glycosylated in transgenic tobacco and maintains its biological activity

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