Object's details: Unusual Zn(II) Affinities of Zinc Fingers of Poly(ADP-ribose) Polymerase 1 (PARP-1) Nuclear Protein

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Unusual Zn(II) Affinities of Zinc Fingers of Poly(ADP-ribose) Polymerase 1 (PARP-1) Nuclear Protein
Creator:
Bossak, Karolina;
Goch, Wojciech;
Piątek, Katarzyna;
Frączyk, Tomasz;
Poznański, Jarosław;
Bonna, Arkadiusz;
Keil, Claudia;
Hartwig, Andrea;
Bal, Wojciech
Description:
Poly(ADP-ribose) polymerase 1 (PARP-1) is a key eukaryotic enzyme, catalyzing the NAD+ dependent poly(ADP-ribosyl)ation of protein substrates, crucial for major DNA repair pathways, and involved in other fundamental cellular processes, such as transcription, cell cycle control, and apoptosis. Its ability to bind DNA depends on two CCHC zinc finger domains, in short, PARPzf1 and PARPzf2. Using spectroscopic methods and competitive titrations with Zn(II), Co(II) and Ni(II) ions we determined conditional dissociation constants for Zn(II) complexes of PARPzf1 and PARPzf2 at pH 7.4 (HEPES buffer), as 26 ± 4 nM and 4 ± 1 pM, respectively. The former value indicates an extremely low affinity of PARPzf1 towards metal ions, meaning that under cellular conditions PARP1zf might be largely present in a “metal-free” state. This finding provides a clue for high susceptibility of PARP-1 to oxidative stress, but also raises questions regarding activation of PARPzf1 under cellular conditions. We also determined conditional dissociation constants for Ni(II) complexes of PARPzf1 and PARPzf2 under the same conditions, as 0.78 ± 0.04 µM and 0.26 ± 0.05 nM, respectively.
Object availability:
free access
Date:
2015.12.30
Type:
Article;
PeerReviewed
Relation: http://eprints.ibb.waw.pl/879 ;http://pubs.acs.org/doi/abs/10.1021/tx500320f
Subject:
Q Science (General);
QD Chemistry;
QH301 Biology;
QP Physiology;
RB Pathology
Identifier:
Bossak, Karolina and Goch, Wojciech and Piątek, Katarzyna and Frączyk, Tomasz and Poznański, Jarosław and Bonna, Arkadiusz and Keil, Claudia and Hartwig, Andrea and Bal, Wojciech (2015) Unusual Zn(II) Affinities of Zinc Fingers of Poly(ADP-ribose) Polymerase 1 (PARP-1) Nuclear Protein. Chemical Research in Toxicology, 28 (2). pp. 191-201. ISSN 0893-228X
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Unusual Zn(II) Affinities of Zinc Fingers of Poly(ADP-ribose) Polymerase 1 (PARP-1) Nuclear Protein

Description

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