Object's details: Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance.
Creator:
Drewniak, Lukasz;
Dziewit, Lukasz;
Ciezkowska, Martyna;
Gawor, Jan;
Gromadka, Robert;
Sklodowska, Aleksandra
Description:
Plasmid pSinA of Sinorhizobium sp. M14 (Alphaproteobacteria) is the first described, natural, self-transferable plasmid harboring a complete set of genes for oxidation of arsenite. Removal of this plasmid from cells of the host strain caused the loss of resistance to arsenic and heavy metals (Cd, Co, Zn and Hg) and abolished the ability to grow on minimal salt medium supplemented with sodium arsenite as the sole energy source. Plasmid pSinA was introduced into other representatives of Alphaproteobacteria which resulted in acquisition of new abilities concerning arsenic resistance and oxidation, as well as heavy metals resistance. Microcosm experiments revealed that plasmid pSinA can also be transferred via conjugation into other indigenous bacteria from microbial community of As-contaminated soils, including representatives of Alpha- and Gammaproteobacteria. Analysis of "natural" transconjugants showed that pSinA is functional (expresses arsenite oxidase) and is stably maintained in their cells after approximately 60 generations of growth under nonselective conditions. This work clearly demonstrates that pSinA is a self-transferable, broad-host-range plasmid, which plays an important role in horizontal transfer of arsenic metabolism genes.
Object availability:
free access
Date:
2013.02.20
Type:
Article;
PeerReviewed
Publisher:
Elsevier B.V.
Relation: http://eprints.ibb.waw.pl/515 ;http://www.journals.elsevier.com/journal-of-biotechnology
Subject:
QH426 Genetics;
QR Microbiology
Identifier:
Drewniak, Lukasz and Dziewit, Lukasz and Ciezkowska, Martyna and Gawor, Jan and Gromadka, Robert and Sklodowska, Aleksandra (2013) Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance. Journal of biotechnology, 164 (4). pp. 479-488. ISSN 1873-4863
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance.

Description

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