Object's details: Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes.
Creator:
Kurzątkowska, Katarzyna;
Mielecki, Marcin;
Grzelak, Krystyna;
Verwilst, Peter;
Dehaen, Wim;
Radecki, Jerzy;
Radecka, Hanna
Description:
New surface plasmon resonance (SPR) sensing platforms which consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His6-JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His6-JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.
Object availability:
free access
Date:
2014.12
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/790 ;http://www.sciencedirect.com/science/article/pii/S0039914014005669
Subject:
QC Physics;
QD Chemistry;
QH301 Biology
Identifier: http://eprints.ibb.waw.pl/790/1/Kurzatkowska2014repozytoriumIBB.pdf ;
Kurzątkowska, Katarzyna and Mielecki, Marcin and Grzelak, Krystyna and Verwilst, Peter and Dehaen, Wim and Radecki, Jerzy and Radecka, Hanna (2014) Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes. Talanta, 130 . pp. 336-41. ISSN 1873-3573
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes.

Description

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