Object's details: Pentetic acid (DPTA) Cu(II) monolayer deposited on gold electrode—The base of biosensors for electrochemical screening of kinase JAK2 and potential inhibitor interactions

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Pentetic acid (DPTA) Cu(II) monolayer deposited on gold electrode—The base of biosensors for electrochemical screening of kinase JAK2 and potential inhibitor interactions
Creator:
Wojtasik, Justyn;
Mielecki, Marcin;
Kurzątkowska, Katarzyna;
Grzelak, Krystyna;
Verwilst, Peter;
Dehaen, Wim;
Radecki, Jerzy;
Radecka, Hanna
Description:
Here, the new biosensor destined for screening of interactions between kinase JAK2 and compounds which may act as inhibitors was presented. The Cu(II) complex of pentetic acid thiol ligand was applied for immobilization of kinase JAK2 on the gold electrode surface through his-tagged chemistry. The base of the biosensor response was the change of the electrochemical properties of the Cu(II) redox centres upon formation of the kinase JAK2–potential inhibitor complex. The increasing inhibitor concentration caused the decrease of reduction/oxidation Cu(II) current observed with Osteryoung square wave voltammetry. The biosensor usability was checked using known inhibitors, berberines–isoquinolone alkaloids, as well as caffeic acid—a control compound which has no affinity to kinase JAK2. The possible parameters suitable for estimation of the strength of the interactions between kinase JAK2 covalently attached to the Cu(II) complex of pentetic acid thiol ligand deposited on the gold electrode surface and potential inhibitors present in the solution were presented.
Object availability:
free access
Date:
2014.06
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/791 ;http://dx.doi.org/10.1016/j.snb.2014.01.095 ;doi:10.1016/j.snb.2014.01.095
Subject:
QC Physics;
QD Chemistry;
QH301 Biology
Identifier: http://eprints.ibb.waw.pl/791/1/Wojtasik2014repozytoriumIBB.pdf ;
Wojtasik, Justyn and Mielecki, Marcin and Kurzątkowska, Katarzyna and Grzelak, Krystyna and Verwilst, Peter and Dehaen, Wim and Radecki, Jerzy and Radecka, Hanna (2014) Pentetic acid (DPTA) Cu(II) monolayer deposited on gold electrode—The base of biosensors for electrochemical screening of kinase JAK2 and potential inhibitor interactions. Sensors and Actuators B: Chemical, 196 . pp. 223-230. ISSN 09254005
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Similar objects

Pentetic acid (DPTA) Cu(II) monolayer deposited on gold electrode—The base of biosensors for electrochemical screening of kinase JAK2 and potential inhibitor interactions

Date:2014.06

Type:other

Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes.

Date:2014.12

Type:other

Determining the Identity of Nucleotides and the Energy of Binding of tRNAs to Their Aminoacyl-tRNA Synthetases Using a Simple Logistic Model

Creator:Pawłowski, Piotr H | Zielenkiewicz, Piotr

Date:2024.10.18

Type:other

The quantum casimir effect may be a universal force organizing the bilayer structure of the cell membrane.

Creator:Pawlowski, Piotr H | Zielenkiewicz, Piotr

Date:2013.05

Type:other

The quantum casimir effect may be a universal force organizing the bilayer structure of the cell membrane.

Creator:Pawlowski, Piotr H | Zielenkiewicz, Piotr

Date:2013.05

Type:other

Theoretical model explaining the relationship between the molecular mass and the activation energy of the enzyme revealed by a large-scale analysis of bioinformatics data.

Creator:Pawlowski, Piotr H | Zielenkiewicz, Piotr

Date:2013

Type:other

Human Annexins A1, A2, and A8 as Potential Molecular Targets for Ni(II) Ions

Date:2014.11.17

Type:other

Zn(II) Complexes of Glutathione Disulfide: Structural Basis of Elevated Stabilities

Creator:Krezel, Artur | Wójcik, Jacek | Maciejczyk, Maciej | Bal, Wojciech

Date:2011

Type:other

Federacja Bibliotek Cyfrowych

Object's details: Pentetic acid (DPTA) Cu(II) monolayer deposited on gold electrode—The base of biosensors for electrochemical screening of kinase JAK2 and potential inhibitor interactions

Description

Similar objects