Object's details: RuvC uses dynamic probing of the Holliday junction to achieve sequence specificity and efficient resolution

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
RuvC uses dynamic probing of the Holliday junction to achieve sequence specificity and efficient resolution
Creator:
Górecka, Karolina Maria;
Krepl, Miroslav;
Szlachcic, Aleksandra;
Poznański, Jarosław;
Šponer, Jiří;
Nowotny, Marcin
Description:
Holliday junctions (HJs) are four-way DNA structures that occur in DNA repair by homologous recombination. Specialized nucleases, termed resolvases, remove (i.e., resolve) HJs. The bacterial protein RuvC is a canonical resolvase that introduces two symmetric cuts into the HJ. For complete resolution of the HJ, the two cuts need to be tightly coordinated. They are also specific for cognate DNA sequences. Using a combination of structural biology, biochemistry, and a computational approach, here we show that correct positioning of the substrate for cleavage requires conformational changes within the bound DNA. These changes involve rare high-energy states with protein-ssisted base flipping that are readily accessible for the cognate DNA sequence but not for non-cognate sequences. These conformational changes and the relief of protein-induced structural tension of the DNA facilitate coordination between the two cuts. The unique DNA cleavage mechanism of RuvC demonstrates the importance of high-energy conformational states in nucleic acid readouts
Object availability:
free access
Rights:
cc_by_nc
Date:
2019
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/1750 ;http://doi.org/10.1038/s41467-019-11900-8 ;doi:10.1038/s41467-019-11900-8
Subject:
Q Science (General)
Identifier: http://eprints.ibb.waw.pl/1750/1/jp2019_NatCom-s41467-019-11900-8.pdf ;
Górecka, Karolina Maria and Krepl, Miroslav and Szlachcic, Aleksandra and Poznański, Jarosław and Šponer, Jiří and Nowotny, Marcin (2019) RuvC uses dynamic probing of the Holliday junction to achieve sequence specificity and efficient resolution. Nature Communications, 10 (1). ISSN 2041-1723
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Federacja Bibliotek Cyfrowych

Object's details: RuvC uses dynamic probing of the Holliday junction to achieve sequence specificity and efficient resolution

Description

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