Object's details: Coordination between the polymerase and RNase H activity of HIV-1 reverse transcriptase

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Coordination between the polymerase and RNase H activity of HIV-1 reverse transcriptase
Creator:
Figiel, Małgorzata;
Krepl, Miroslav;
Poznański, Jarosław;
Gołąb, Agnieszka;
Šponer, Jiří;
Nowotny, Marcin
Description:
Replication of human immunodeficiency virus 1 (HIV-1) involves conversion of its single-stranded RNA genome to double-stranded DNA, which is integrated into the genome of the host. This conversion is catalyzed by reverse transcriptase (RT), which possesses DNA polymerase and RNase H domains. The available crystal structures suggest that at any given time the RNA/DNA substrate interacts with only one active site of the two domains of HIV-1 RT. Unknown is whether a simultaneous interaction of the substrate with polymerase and RNase H active sites is possible. Therefore, the mechanism of the coordination of the two activities is not fully understood. We performed molecular dynamics simulations to obtain a conformation of the complex in which the unwound RNA/DNA substrate simultaneously interacts with the polymerase and RNase H active sites. When the RNA/DNA hybrid was immobilized at the polymerase active site, RNase H cleavage occurred, experimentally verifying that the substrate can simultaneously interact with both active sites. These findings demonstrate the existence of a transient conformation of the HIV-1 RT substrate complex, which is important for modulating and coordinating the enzymatic activities of HIV-1 RT.
Object availability:
free access
Date:
2017.04.07
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
Oxford University Press
Relation: http://eprints.ibb.waw.pl/1328 ;http://doi.org/10.1093/nar/gkx004 ;doi:10.1093/nar/gkx004
Subject:
Q Science (General)
Identifier: http://eprints.ibb.waw.pl/1328/1/jp2017_NAR-gkx004.pdf ;
Figiel, Małgorzata and Krepl, Miroslav and Poznański, Jarosław and Gołąb, Agnieszka and Šponer, Jiří and Nowotny, Marcin (2017) Coordination between the polymerase and RNase H activity of HIV-1 reverse transcriptase. Nucleic Acids Research, 45 (6). pp. 3341-3352. ISSN 0305-1048
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Coordination between the polymerase and RNase H activity of HIV-1 reverse transcriptase

Description

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