Szczegóły obiektu: Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization

Instytucja dostarczająca:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Opis

Tytuł:
Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization
Twórca:
Fernández-Bachiller, Maria Isabel;
Brzozowska, Iwona;
Odolczyk, Norbert;
Zielenkiewicz, Urszula;
Zielenkiewicz, Piotr;
Rademann, Jörg
Opis:
Toxin–antitoxin systems constitute a native survival strategy of pathogenic bacteria and thus are potential targets of antibiotic drugs. Here, we target the Zeta–Epsilon toxin–antitoxin system, which is responsible for the stable maintenance of certain multiresistance plasmids in Gram-positive bacteria. Peptide ligands were designed on the basis of the ε2ζ2 complex. Three α helices of Zeta forming the protein–protein interaction (PPI) site were selected and peptides were designed conserving the residues interacting with Epsilon antitoxin while substituting residues binding intramolecularly to other parts of Zeta. Designed peptides were synthesized with an N-terminal fluoresceinyl-carboxy-residue for binding assays and provided active ligands, which were used to define the hot spots of the ε2ζ2 complex. Further shortening and modification of the binding peptides provided ligands with affinities <100 nM, allowing us to determine the most relevant PPIs and implement a robust competition binding assay.
Dostępność obiektu:
Dostęp otwarty
Data:
2016.07.16
Typ:
Article;
PeerReviewed
Język:
angielski;
eng
Format:
application/pdf
Wydawca:
Multidisciplinary Digital Publishing Institute
Relacja: http://eprints.ibb.waw.pl/1206 ;http://www.mdpi.com/2072-6651/8/7/222
Temat i słowa kluczowe:
QD Chemistry;
QR Microbiology
Identyfikator: http://eprints.ibb.waw.pl/1206/1/toxins-08-00222.pdf ;
Fernández-Bachiller, Maria Isabel and Brzozowska, Iwona and Odolczyk, Norbert and Zielenkiewicz, Urszula and Zielenkiewicz, Piotr and Rademann, Jörg (2016) Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization. Toxins, 8 (222).
Dostawca danych:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Szczegóły obiektu: Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization

Opis

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