Opis

• Tytuł:
  Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT.
• Twórca:
  Chen, Emily;
  Kiebish, Michael;
  McDaniel, Justice;
  Niedźwiecka, Katarzyna;
  Kucharczyk, Roza;
  Ravasz, Dora;
  Gao, Fei;
  Narain, Niven;
  Sarangarajan, Rangaprasad;
  Seyfield, Thomas;
  Adam-Vizi, Vera;
  Chinopoulos, Christos
• Opis:
  Heterologous expression is a landmark technique for studying a protein itself or its effect on the expression host, in which membrane-embedded proteins are a common choice. Yet, the impact of inserting a foreign protein to the lipid environment of host membranes, has never been addressed. Here we demonstrated that heterologous expression of the Artemia franciscana adenine nucleotide translocase (ANT) in yeasts altered lipidomic composition of their inner mitochondrial membranes. Along with this, activities of complex II, IV and ATP synthase, all membrane-embedded components, were significantly decreased while their expression levels remained unaffected. Although the results represent an individual case of expressing a crustacean protein in yeast inner mitochondrial membranes, it cannot be excluded that host lipidome alterations is a more widespread epiphenomenon, potentially biasing heterologous expression experiments. Finally, our results raise the possibility that not only lipids modulate protein function, but also membrane-embedded proteins modulate lipid composition, thus revealing a reciprocal mode of regulation for these two biomolecular entities.
• Dostępność obiektu:
  Dostęp otwarty
• Data:
  2018.04
• Typ:
  Article;
  PeerReviewed
• Język:
  angielski;
  eng
• Format:
  application/pdf
• Relacja: http://eprints.ibb.waw.pl/1557 ;https://www.nature.com/articles/s41598-018-24305-2 ;
  10.1038/s41598-018-24305-2.
• Temat i słowa kluczowe:
  QD Chemistry;
  QR Microbiology
• Identyfikator: http://eprints.ibb.waw.pl/1557/1/Chen-SciRep2018.pdf ;
  Chen, Emily and Kiebish, Michael and McDaniel, Justice and Niedźwiecka, Katarzyna and Kucharczyk, Roza and Ravasz, Dora and Gao, Fei and Narain, Niven and Sarangarajan, Rangaprasad and Seyfield, Thomas and Adam-Vizi, Vera and Chinopoulos, Christos (2018) Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT. Scientific Reports, 8 (5915). ISSN 2045-2322
• Dostawca danych:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
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