Object's details: Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization
Creator:
Fernández-Bachiller, Maria Isabel;
Brzozowska, Iwona;
Odolczyk, Norbert;
Zielenkiewicz, Urszula;
Zielenkiewicz, Piotr;
Rademann, Jörg
Description:
Toxin–antitoxin systems constitute a native survival strategy of pathogenic bacteria and thus are potential targets of antibiotic drugs. Here, we target the Zeta–Epsilon toxin–antitoxin system, which is responsible for the stable maintenance of certain multiresistance plasmids in Gram-positive bacteria. Peptide ligands were designed on the basis of the ε2ζ2 complex. Three α helices of Zeta forming the protein–protein interaction (PPI) site were selected and peptides were designed conserving the residues interacting with Epsilon antitoxin while substituting residues binding intramolecularly to other parts of Zeta. Designed peptides were synthesized with an N-terminal fluoresceinyl-carboxy-residue for binding assays and provided active ligands, which were used to define the hot spots of the ε2ζ2 complex. Further shortening and modification of the binding peptides provided ligands with affinities <100 nM, allowing us to determine the most relevant PPIs and implement a robust competition binding assay.
Object availability:
free access
Date:
2016.07.16
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Publisher:
Multidisciplinary Digital Publishing Institute
Relation: http://eprints.ibb.waw.pl/1206 ;http://www.mdpi.com/2072-6651/8/7/222
Subject:
QD Chemistry;
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/1206/1/toxins-08-00222.pdf ;
Fernández-Bachiller, Maria Isabel and Brzozowska, Iwona and Odolczyk, Norbert and Zielenkiewicz, Urszula and Zielenkiewicz, Piotr and Rademann, Jörg (2016) Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization. Toxins, 8 (222).
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Mapping Protein–Protein Interactions of the Resistance-Related Bacterial Zeta Toxin–Epsilon Antitoxin Complex (ε2ζ2) with High Affinity Peptide Ligands Using Fluorescence Polarization

Description

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