Object's details: Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT.
Creator:
Chen, Emily;
Kiebish, Michael;
McDaniel, Justice;
Niedźwiecka, Katarzyna;
Kucharczyk, Roza;
Ravasz, Dora;
Gao, Fei;
Narain, Niven;
Sarangarajan, Rangaprasad;
Seyfield, Thomas;
Adam-Vizi, Vera;
Chinopoulos, Christos
Description:
Heterologous expression is a landmark technique for studying a protein itself or its effect on the expression host, in which membrane-embedded proteins are a common choice. Yet, the impact of inserting a foreign protein to the lipid environment of host membranes, has never been addressed. Here we demonstrated that heterologous expression of the Artemia franciscana adenine nucleotide translocase (ANT) in yeasts altered lipidomic composition of their inner mitochondrial membranes. Along with this, activities of complex II, IV and ATP synthase, all membrane-embedded components, were significantly decreased while their expression levels remained unaffected. Although the results represent an individual case of expressing a crustacean protein in yeast inner mitochondrial membranes, it cannot be excluded that host lipidome alterations is a more widespread epiphenomenon, potentially biasing heterologous expression experiments. Finally, our results raise the possibility that not only lipids modulate protein function, but also membrane-embedded proteins modulate lipid composition, thus revealing a reciprocal mode of regulation for these two biomolecular entities.
Object availability:
free access
Date:
2018.04
Type:
Article;
PeerReviewed
Language:
English;
eng
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/1557 ;https://www.nature.com/articles/s41598-018-24305-2 ;
10.1038/s41598-018-24305-2.
Subject:
QD Chemistry;
QR Microbiology
Identifier: http://eprints.ibb.waw.pl/1557/1/Chen-SciRep2018.pdf ;
Chen, Emily and Kiebish, Michael and McDaniel, Justice and Niedźwiecka, Katarzyna and Kucharczyk, Roza and Ravasz, Dora and Gao, Fei and Narain, Niven and Sarangarajan, Rangaprasad and Seyfield, Thomas and Adam-Vizi, Vera and Chinopoulos, Christos (2018) Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT. Scientific Reports, 8 (5915). ISSN 2045-2322
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT.

Description

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