Description

• Title:
  Effect of surfactants on surface activity and rheological properties of type I collagen at air/water interface
• Creator:
  Kezwoń, Aleksandra;
  Góral, Ilona;
  Frączyk, Tomasz;
  Wojciechowski, Kamil
• Description:
  We describe the effect of three synthetic surfactants (anionic - sodium dodecyl sulfate (SDS), cationic - cetyltrimethylammonium bromide (CTAB) and nonionic - Triton X-100 (TX-100)) on surface properties of the type I calf skin collagen at the air/water interface in acidic solutions (pH 1.8). The protein concentration was fixed at 5×10-6molL-1 and the surfactant concentration was varied in the range 5×10-6molL-1-1×10-4molL-1, producing the protein/surfactant mixtures with molar ratios of 1:1, 1:2, 1:5, 1:10 and 1:20. An Axisymmetric Drop Shape Analysis (ADSA) method was used to determine the dynamic surface tension and surface dilatational moduli of the mixed adsorption layers. Two spectroscopic techniques: UV-vis spectroscopy and fluorimetry allowed us to determine the effect of the surfactants on the protein structure. The thermodynamic characteristic of the mixtures was studied using isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC). Modification of the collagen structure by SDS at low surfactant/protein ratios has a positive effect on the mixture's surface activity with only minor deterioration of the rheological properties of the adsorbed layers. The collagen/CTAB mixtures do not show that pronounced improvement in surface activity, while rheological properties are significantly deteriorated. The mixtures with non-ionic TX-100 do not show any synergistic effects in surface activity.
• Object availability:
  free access
• Date:
  2016
• Type:
  Article;
  PeerReviewed
• Language:
  English;
  eng
• Format:
  application/msword
• Publisher:
  Elsevier
• Relation: http://eprints.ibb.waw.pl/1238 ;http://www.sciencedirect.com/science/article/pii/S092777651630635X
• Subject:
  QD Chemistry;
  QH301 Biology
• Identifier: http://eprints.ibb.waw.pl/1238/1/Effect_of_surfactants_on_collagen_2016.docx ;
  Kezwoń, Aleksandra and Góral, Ilona and Frączyk, Tomasz and Wojciechowski, Kamil (2016) Effect of surfactants on surface activity and rheological properties of type I collagen at air/water interface. Colloids and surfaces B: Biointerfaces, 148 . pp. 238-248. ISSN 0927-7765
• Data provider:
  Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk
• Can I use it?:
  seek permission
• Type:
  other