Object's details: Electrochemical Detection of Avian Influenza Virus Genotype Using Amino-ssDNA Probe Modified Gold Electrodes

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
Electrochemical Detection of Avian Influenza Virus Genotype Using Amino-ssDNA Probe Modified Gold Electrodes
Creator:
Malecka, Kamila;
Grabowska, Iwona;
Radecki, Jerzy;
Stachyra, Anna;
Góra-Sochacka, Anna;
Sirko, Agnieszka;
Radecka, Hanna
Description:
A DNA biosensor for the detection of specific oligonucleotide sequences of Avian Influenza Virus type H5N1 has been proposed. The NH2-ssDNA probe was deposited onto a gold electrode surface to form an amide bond between the carboxyl group of thioacid and the amino group from ssDNA probe. The signals generated as a result of hybridization were registered in square wave voltammetry and electrochemical impedance spectroscopy in the presence of [Fe(CN)6]3�/4� as a redox marker. The genosensor is capable to determine 20-mer and 180-bp (PCR products) oligonucleotides complementary sequences with detection limit in the fM range. The genosensor displays good selectivity and sensitivity. The 20-mer as well as 180-bp oligonucleotides without a complementary sequence generate very low signal.
Object availability:
free access
Date:
2013.07.16
Type:
Article;
PeerReviewed
Publisher:
WILEY-VCH
Relation: http://eprints.ibb.waw.pl/589 ;http://onlinelibrary.wiley.com/doi/10.1002/elan.201300113/pdf ;DOI: 10.1002/elan.201300113
Subject:
QD Chemistry;
TP Chemical technology
Identifier:
Malecka, Kamila and Grabowska, Iwona and Radecki, Jerzy and Stachyra, Anna and Góra-Sochacka, Anna and Sirko, Agnieszka and Radecka, Hanna (2013) Electrochemical Detection of Avian Influenza Virus Genotype Using Amino-ssDNA Probe Modified Gold Electrodes. Electroanalysis, 25 (8). pp. 1871-1878. ISSN 1040-0397
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: Electrochemical Detection of Avian Influenza Virus Genotype Using Amino-ssDNA Probe Modified Gold Electrodes

Description

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