Object's details: An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin h5.

Provider:Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

Description

Title:
An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin h5.
Creator:
Jarocka, Urszula;
Sawicka, Róża;
Góra-Sochacka, Anna;
Sirko, Agnieszka;
Zagórski-Ostoja, Włodzimierz;
Radecki, Jerzy;
Radecka, Hanna
Description:
This paper concerns the development of an immunosensor for detection of peptides derived from avian influenza hemagglutinin H5. Its preparation consists of successive gold electrode modification steps: (i) modification with 1,6-hexanedithiol and gold colloidal nanoparticles; (ii) immobilization of antibody-binding fragments (Fab') of anti-hemagglutinin H5 monoclonal antibodies Mab 6-9-1 via S-Au covalent bonds; and (iii) covering the remaining free space on the electrode surfaces with bovine serum albumin. The interactions between Fab' fragments and hemagglutinin (HA) variants have been explored with electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)6]3-/4- as an electroactive marker. The immunosensor was able to recognize three different His-tagged variants of recombinant hemagglutinin from H5N1 viruses: H1 subunit (17-340 residues) of A/swan/Poland/305-135V08/2006, the long HA (17-530 residues) A/Bar-headed Goose/Qinghai/12/2005 and H1 subunit (1-345 residues) of A/Vietnam/1194/2004. The strongest response has been observed for the long variant with detection limit of 2.2 pg/mL and dynamic range from 4.0 to 20.0 pg/mL.
Object availability:
free access
Date:
2014
Type:
Article;
PeerReviewed
Language:
English
Format:
application/pdf
Relation: http://eprints.ibb.waw.pl/756 ;http://www.mdpi.com/journal/sensors ;doi:10.3390/s140915714
Subject:
QD Chemistry
Identifier: http://eprints.ibb.waw.pl/756/1/jarocka-2014-sensors-14-15714.pdf ;
Jarocka, Urszula and Sawicka, Róża and Góra-Sochacka, Anna and Sirko, Agnieszka and Zagórski-Ostoja, Włodzimierz and Radecki, Jerzy and Radecka, Hanna (2014) An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin h5. Sensors (Basel, Switzerland), 14 (9). pp. 15714-28. ISSN 1424-8220
Data provider:
Repozytorium Instytutu Biochemii i Biofizyki Polskiej Akademii Nauk

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Object's details: An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin h5.

Description

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